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| | <StructureSection load='6nce' size='340' side='right'caption='[[6nce]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='6nce' size='340' side='right'caption='[[6nce]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nce]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NCE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nce]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NCE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.598Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FOXN3, C14orf116, CHES1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nce OCA], [http://pdbe.org/6nce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nce RCSB], [http://www.ebi.ac.uk/pdbsum/6nce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nce ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nce OCA], [https://pdbe.org/6nce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nce RCSB], [https://www.ebi.ac.uk/pdbsum/6nce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nce ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FOXN3_HUMAN FOXN3_HUMAN]] Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).<ref>PMID:16102918</ref> | + | [https://www.uniprot.org/uniprot/FOXN3_HUMAN FOXN3_HUMAN] Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).<ref>PMID:16102918</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Blacklow, S C]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Bulyk, M L]] | + | [[Category: Blacklow SC]] |
| - | [[Category: Hallworth, A N]] | + | [[Category: Bulyk ML]] |
| - | [[Category: Jarrett, S M]] | + | [[Category: Hallworth AN]] |
| - | [[Category: Rogers, J M]] | + | [[Category: Jarrett SM]] |
| - | [[Category: Seegar, T C]] | + | [[Category: Rogers JM]] |
| - | [[Category: Waters, C T]] | + | [[Category: Seegar TC]] |
| - | [[Category: Dna binding protein]]
| + | [[Category: Waters CT]] |
| - | [[Category: Dna binding protein-dna complex]]
| + | |
| - | [[Category: Dna binding transcription factor activity]]
| + | |
| - | [[Category: Forkhead]]
| + | |
| - | [[Category: Regulation of transcription dna templated]]
| + | |
| - | [[Category: Sequence specific dna binding]]
| + | |
| Structural highlights
Function
FOXN3_HUMAN Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).[1]
Publication Abstract from PubMed
Transcription factors (TFs) control gene expression by binding DNA recognition sites in genomic regulatory regions. Although most forkhead TFs recognize a canonical forkhead (FKH) motif, RYAAAYA, some forkheads recognize a completely different (FHL) motif, GACGC. Bispecific forkhead proteins recognize both motifs, but the molecular basis for bispecific DNA recognition is not understood. We present co-crystal structures of the FoxN3 DNA binding domain bound to the FKH and FHL sites, respectively. FoxN3 adopts a similar conformation to recognize both motifs, making contacts with different DNA bases using the same amino acids. However, the DNA structure is different in the two complexes. These structures reveal how a single TF binds two unrelated DNA sequences and the importance of DNA shape in the mechanism of bispecific recognition.
Bispecific Forkhead Transcription Factor FoxN3 Recognizes Two Distinct Motifs with Different DNA Shapes.,Rogers JM, Waters CT, Seegar TCM, Jarrett SM, Hallworth AN, Blacklow SC, Bulyk ML Mol Cell. 2019 Feb 22. pii: S1097-2765(19)30039-5. doi:, 10.1016/j.molcel.2019.01.019. PMID:30826165[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Scott KL, Plon SE. CHES1/FOXN3 interacts with Ski-interacting protein and acts as a transcriptional repressor. Gene. 2005 Oct 10;359:119-26. PMID:16102918 doi:http://dx.doi.org/S0378-1119(05)00364-1
- ↑ Rogers JM, Waters CT, Seegar TCM, Jarrett SM, Hallworth AN, Blacklow SC, Bulyk ML. Bispecific Forkhead Transcription Factor FoxN3 Recognizes Two Distinct Motifs with Different DNA Shapes. Mol Cell. 2019 Feb 22. pii: S1097-2765(19)30039-5. doi:, 10.1016/j.molcel.2019.01.019. PMID:30826165 doi:http://dx.doi.org/10.1016/j.molcel.2019.01.019
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