|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6njq' size='340' side='right'caption='[[6njq]], [[Resolution|resolution]] 2.75Å' scene=''> | | <StructureSection load='6njq' size='340' side='right'caption='[[6njq]], [[Resolution|resolution]] 2.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6njq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NJQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6njq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NJQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TBP1, At3g13445, MRP15.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6njq OCA], [http://pdbe.org/6njq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6njq RCSB], [http://www.ebi.ac.uk/pdbsum/6njq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6njq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6njq OCA], [https://pdbe.org/6njq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6njq RCSB], [https://www.ebi.ac.uk/pdbsum/6njq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6njq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TBP1_ARATH TBP1_ARATH]] General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. | + | [https://www.uniprot.org/uniprot/TBP1_ARATH TBP1_ARATH] General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Schumacher, M A]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Stelling, A]] | + | [[Category: Schumacher MA]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Stelling A]] |
| - | [[Category: Dna binding protein-dna complex]]
| + | |
| - | [[Category: Hoogsteen]]
| + | |
| - | [[Category: Protein-dna]]
| + | |
| - | [[Category: Tbp]]
| + | |
| Structural highlights
Function
TBP1_ARATH General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Publication Abstract from PubMed
Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C(+) ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C(+) Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes.
Infrared Spectroscopic Observation of a G-C(+) Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.,Stelling AL, Liu AY, Zeng W, Salinas R, Schumacher MA, Al-Hashimi HM Angew Chem Int Ed Engl. 2019 Aug 26;58(35):12010-12013. doi:, 10.1002/anie.201902693. Epub 2019 Jul 25. PMID:31268220[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Stelling AL, Liu AY, Zeng W, Salinas R, Schumacher MA, Al-Hashimi HM. Infrared Spectroscopic Observation of a G-C(+) Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions. Angew Chem Int Ed Engl. 2019 Aug 26;58(35):12010-12013. doi:, 10.1002/anie.201902693. Epub 2019 Jul 25. PMID:31268220 doi:http://dx.doi.org/10.1002/anie.201902693
|
