|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6ow4' size='340' side='right'caption='[[6ow4]], [[Resolution|resolution]] 1.99Å' scene=''> | | <StructureSection load='6ow4' size='340' side='right'caption='[[6ow4]], [[Resolution|resolution]] 1.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ow4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_adolescentis_l2-32 Bifidobacterium adolescentis l2-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OW4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ow4]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_adolescentis_L2-32 Bifidobacterium adolescentis L2-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OW4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BIFADO_01909 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=411481 Bifidobacterium adolescentis L2-32])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ow4 OCA], [http://pdbe.org/6ow4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ow4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ow4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ow4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ow4 OCA], [https://pdbe.org/6ow4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ow4 RCSB], [https://www.ebi.ac.uk/pdbsum/6ow4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ow4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A7A7R9_BIFAD A7A7R9_BIFAD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 17: |
Line 19: |
| | </div> | | </div> |
| | <div class="pdbe-citations 6ow4" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6ow4" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Hydroxysteroid dehydrogenase 3D structures|Hydroxysteroid dehydrogenase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bifidobacterium adolescentis l2-32]] | + | [[Category: Bifidobacterium adolescentis L2-32]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Koropatkin, N M]] | + | [[Category: Koropatkin NM]] |
| - | [[Category: Mythen, S M]] | + | [[Category: Mythen SM]] |
| - | [[Category: Pollet, R M]] | + | [[Category: Pollet RM]] |
| - | [[Category: Ridlon, J M]] | + | [[Category: Ridlon JM]] |
| - | [[Category: Nadh dependent]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Pyridine nucleotide-dependent enzyme]]
| + | |
| - | [[Category: Short-chain dehydrogenase/reductase]]
| + | |
| Structural highlights
Function
A7A7R9_BIFAD
Publication Abstract from PubMed
Anaerobic bacteria inhabiting the human gastrointestinal tract have evolved various enzymes that modify host-derived steroids. The bacterial steroid-17,20-desmolase pathway cleaves the cortisol side-chain, forming pro-androgens predicted to impact host physiology. Bacterial 20beta-hydroxysteroid dehydrogenase (20beta-HSDH) regulates cortisol side-chain cleavage by reducing the C-20 carboxyl group on cortisol, yielding 20beta-dihydrocortisol. Recently, the gene encoding 20beta-HSDH in Butyricicoccus desmolans ATCC 43058 was reported, and a non-redundant protein search yielded a candidate 20beta-HSDH gene in Bifidobacterium adolescentis strain L2-32. B. adolescentis 20beta-HSDH could regulate cortisol side-chain cleavage by limiting pro-androgen formation in bacteria such as Clostridium scindens and 21-dehydroxylation by Eggerthella lenta Here, the putative B. adolescentis 20beta-HSDH was cloned, overexpressed and purified. 20beta-HSDH activity was confirmed through whole cell and pure enzymatic assays and it is specific for cortisol. Next, we solved the structures of recombinant 20beta-HSDH in both the apo and holo forms at 2.0-2.2A resolutions, revealing close overlap except for rearrangements near the active site. Interestingly, the structures contain a large, flexible N-terminal region which was investigated by gel filtration chromatography and CD spectroscopy. This extended N-terminus is important for protein stability because deletions of varying lengths caused structural changes and reduced enzymatic activity. A nonconserved extended N-terminus was also observed in several short-chain dehydro-genase/reductase family members. B. adolescentis strains capable of 20beta-HSDH activity could alter glucocorticoid metabolism in the gut and thereby serve as potential probiotics for the management of androgen-dependent diseases.
Structural and biochemical characterization of 20beta-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32.,Doden HL, Pollet RM, Mythen SM, Wawrzak Z, Devendran S, Cann I, Koropatkin NM, Ridlon JM J Biol Chem. 2019 Jun 17. pii: RA119.009390. doi: 10.1074/jbc.RA119.009390. PMID:31209107[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Doden HL, Pollet RM, Mythen SM, Wawrzak Z, Devendran S, Cann I, Koropatkin NM, Ridlon JM. Structural and biochemical characterization of 20beta-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32. J Biol Chem. 2019 Jun 17. pii: RA119.009390. doi: 10.1074/jbc.RA119.009390. PMID:31209107 doi:http://dx.doi.org/10.1074/jbc.RA119.009390
|
