1nf3
From Proteopedia
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'''Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6''' | '''Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6''' | ||
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[[Category: Rosen, M K.]] | [[Category: Rosen, M K.]] | ||
[[Category: Tomchick, D R.]] | [[Category: Tomchick, D R.]] | ||
| - | [[Category: | + | [[Category: Gtpase binding domain]] |
| - | [[Category: | + | [[Category: Pdz domain]] |
| - | [[Category: | + | [[Category: Semi-crib motif]] |
| - | [[Category: | + | [[Category: Switch i and ii]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:27:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:27, 2 May 2008
Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6
Overview
Cdc42 is a small GTPase that is required for cell polarity establishment in eukaryotes as diverse as budding yeast and mammals. Par6 is also implicated in metazoan cell polarity establishment and asymmetric cell divisions. Cdc42.GTP interacts with proteins that contain a conserved sequence called a CRIB motif. Uniquely, Par6 possesses a semi-CRIB motif that is not sufficient for binding to Cdc42. An adjacent PDZ domain is also necessary and is required for biological effects of Par6. Here we report the crystal structure of a complex between Cdc42 and the Par6 GTPase-binding domain. The semi-CRIB motif forms a beta-strand that inserts between the four strands of Cdc42 and the three strands of the PDZ domain to form a continuous eight-stranded sheet. Cdc42 induces a conformational change in Par6, detectable by fluorescence resonance energy transfer spectroscopy. Nuclear magnetic resonance studies indicate that the semi-CRIB motif of Par6 is at least partially structured by the PDZ domain. The structure highlights a novel role for a PDZ domain as a structural scaffold.
About this Structure
1NF3 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6., Garrard SM, Capaldo CT, Gao L, Rosen MK, Macara IG, Tomchick DR, EMBO J. 2003 Mar 3;22(5):1125-33. PMID:12606577 Page seeded by OCA on Sat May 3 02:27:22 2008
