1nf6
From Proteopedia
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'''X-ray structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different catalytic states ("cycled" structure: reduced in solution and allowed to reoxidise before crystallisation)''' | '''X-ray structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different catalytic states ("cycled" structure: reduced in solution and allowed to reoxidise before crystallisation)''' | ||
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[[Category: Xavier, A V.]] | [[Category: Xavier, A V.]] | ||
[[Category: 24 subunits in the active molecule]] | [[Category: 24 subunits in the active molecule]] | ||
| - | [[Category: | + | [[Category: Bacterioferritin]] |
| - | [[Category: | + | [[Category: Diiron centre]] |
| - | [[Category: | + | [[Category: Haem fe-coproporphyrin iii cofactor]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:27:38 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:27, 2 May 2008
X-ray structure of the Desulfovibrio desulfuricans bacterioferritin: the diiron site in different catalytic states ("cycled" structure: reduced in solution and allowed to reoxidise before crystallisation)
Overview
The first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. The protein, isolated from the anaerobic bacterium Desulfovibrio desulfuricans, has the unique property of having Fe-coproporphyrin III as its heme cofactor. The three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of the di-iron ferroxidase site. Conformational changes associated with these intermediates support the idea of a route for iron entry into the protein shell through a pore that passes through the di-iron center. Molecular surface and electrostatic potential calculations also suggest the presence of another ion channel, distant from the channels at the three- and four-fold axes proposed as points of entry for the iron atoms.
About this Structure
1NF6 is a Single protein structure of sequence from Desulfovibrio desulfuricans. Full crystallographic information is available from OCA.
Reference
The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricans., Macedo S, Romao CV, Mitchell E, Matias PM, Liu MY, Xavier AV, LeGall J, Teixeira M, Lindley P, Carrondo MA, Nat Struct Biol. 2003 Apr;10(4):285-90. PMID:12627224 Page seeded by OCA on Sat May 3 02:27:38 2008
Categories: Desulfovibrio desulfuricans | Single protein | Carrondo, M A. | LeGall, J. | Lindley, P. | Liu, M Y. | Macedo, S. | Matias, P M. | Mitchell, E. | Romao, C V. | Teixeira, M. | Xavier, A V. | 24 subunits in the active molecule | Bacterioferritin | Diiron centre | Haem fe-coproporphyrin iii cofactor
