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| | <StructureSection load='6u8c' size='340' side='right'caption='[[6u8c]], [[Resolution|resolution]] 2.61Å' scene=''> | | <StructureSection load='6u8c' size='340' side='right'caption='[[6u8c]], [[Resolution|resolution]] 2.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6u8c]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Strsg Strsg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U8C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U8C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6u8c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U8C FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1320 STRSG])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.61Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u8c OCA], [http://pdbe.org/6u8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u8c RCSB], [http://www.ebi.ac.uk/pdbsum/6u8c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u8c ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u8c OCA], [https://pdbe.org/6u8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u8c RCSB], [https://www.ebi.ac.uk/pdbsum/6u8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u8c ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SPG2_STRSG SPG2_STRSG] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| | + | *[[Antibody 3D structures|Antibody 3D structures]] |
| | *[[Protein G|Protein G]] | | *[[Protein G|Protein G]] |
| | + | *[[3D structures of human antibody|3D structures of human antibody]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strsg]] | + | [[Category: Streptococcus sp. 'group G']] |
| - | [[Category: Davydova, E K]] | + | [[Category: Davydova EK]] |
| - | [[Category: Filippova, E V]] | + | [[Category: Filippova EV]] |
| - | [[Category: Kossiakoff, A A]] | + | [[Category: Kossiakoff AA]] |
| - | [[Category: Slezak, T]] | + | [[Category: Slezak T]] |
| - | [[Category: Engineered fab]]
| + | |
| - | [[Category: High affinity]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Protein g]]
| + | |
| Structural highlights
Function
SPG2_STRSG
Publication Abstract from PubMed
Engineered recombinant antibody-based reagents are rapidly supplanting traditionally derived antibodies in many cell biological applications. A particularly powerful aspect of these engineered reagents is that other modules having myriad functions can be attached to them either chemically or through molecular fusions. However, these processes can be cumbersome and do not lend themselves to high throughput applications. Consequently, we have endeavored to develop a platform that can introduce multiple functionalities into a class of Fab-based affinity reagents in a "plug and play" fashion. This platform exploits the ultra-tight binding interaction between affinity matured variants of a Fab scaffold (Fab(S) ) and a domain of an immunoglobulin binding protein, protein G (GA1). GA1 is easily genetically manipulatable facilitating the ability to link these modules together like beads on a string with adjustable spacing to produce multivalent and bi-specific entities. GA1 can also be fused to other proteins or be chemically modified to engage other types of functional components. To demonstrate the utility for the Fab-GA1 platform, we applied it to a detection proximity assay based on the beta-lactamase (BL) split enzyme system. We also show the bi-specific capabilities of the module by using it in context of a Bi-specific T-cell engager (BiTE), which is a therapeutic assemblage that induces cell killing by crosslinking T-cells to cancer cells. We show that GA1-Fab modules are easily engineered into potent cell killing BiTE-like assemblages and have the advantage of interchanging Fabs directed against different cell surface cancer related targets in a plug and play fashion. This article is protected by copyright. All rights reserved.
An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability.,Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA Protein Sci. 2019 Oct 17. doi: 10.1002/pro.3751. PMID:31622515[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Slezak T, Bailey LJ, Jaskolowski M, Nahotko DA, Filippova EV, Davydova EK, Kossiakoff AA. An engineered ultra-high affinity Fab-Protein G pair enables a modular antibody platform with multifunctional capability. Protein Sci. 2019 Oct 17. doi: 10.1002/pro.3751. PMID:31622515 doi:http://dx.doi.org/10.1002/pro.3751
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