6udn
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Crystal Structure of a Self-Assembling DNA Scaffold Containing TA Sticky Ends and Rhombohedral Symmetry== |
| - | <StructureSection load='6udn' size='340' side='right'caption='[[6udn]]' scene=''> | + | <StructureSection load='6udn' size='340' side='right'caption='[[6udn]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6udn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UDN FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6udn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6udn OCA], [https://pdbe.org/6udn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6udn RCSB], [https://www.ebi.ac.uk/pdbsum/6udn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6udn ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.602Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARS:ARSENIC'>ARS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6udn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6udn OCA], [https://pdbe.org/6udn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6udn RCSB], [https://www.ebi.ac.uk/pdbsum/6udn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6udn ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson-Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high-resolution (2.7 A) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 A) using a modified sticky-end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 A resolution by molecular replacement. | ||
| + | |||
| + | A Self-Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High-Resolution Structural Detail.,Simmons CR, MacCulloch T, Zhang F, Liu Y, Stephanopoulos N, Yan H Angew Chem Int Ed Engl. 2020 Oct 12;59(42):18619-18626. doi: , 10.1002/anie.202005505. Epub 2020 Aug 17. PMID:32533629<ref>PMID:32533629</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6udn" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Synthetic construct]] |
| + | [[Category: MacCulloch T]] | ||
| + | [[Category: Simmons CR]] | ||
| + | [[Category: Stephanopoulos N]] | ||
| + | [[Category: Yan H]] | ||
Current revision
Crystal Structure of a Self-Assembling DNA Scaffold Containing TA Sticky Ends and Rhombohedral Symmetry
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