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| | <StructureSection load='6v1d' size='340' side='right'caption='[[6v1d]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='6v1d' size='340' side='right'caption='[[6v1d]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6v1d]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V1D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V1D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6v1d]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V1D FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TFF1, BCEI, PS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v1d OCA], [http://pdbe.org/6v1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v1d RCSB], [http://www.ebi.ac.uk/pdbsum/6v1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v1d ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v1d OCA], [https://pdbe.org/6v1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v1d RCSB], [https://www.ebi.ac.uk/pdbsum/6v1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v1d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TFF1_HUMAN TFF1_HUMAN]] Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.<ref>PMID:16308573</ref> | + | [https://www.uniprot.org/uniprot/TFF1_HUMAN TFF1_HUMAN] Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.<ref>PMID:16308573</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Goddard-Borger, E D]] | + | [[Category: Goddard-Borger ED]] |
| - | [[Category: Jarva, M A]] | + | [[Category: Jarva MA]] |
| - | [[Category: John, A]] | + | [[Category: John A]] |
| - | [[Category: Lingford, J P]] | + | [[Category: Lingford JP]] |
| - | [[Category: Scott, N E]] | + | [[Category: Scott NE]] |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Mucin binding protein]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| - | [[Category: Trefoil factor]]
| + | |
| Structural highlights
Function
TFF1_HUMAN Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.[1]
Publication Abstract from PubMed
The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs). The TFFs increase the viscoelasticity of the mucosa and promote cell migration, though the molecular mechanisms underlying these functions have remained poorly defined. Here, we demonstrate that all TFFs are divalent lectins that recognise the GlcNAc-alpha-1,4-Gal disaccharide, which terminates some mucin-like O-glycans. Degradation of this disaccharide by a glycoside hydrolase abrogates TFF binding to mucins. Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges. These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.
Trefoil factors share a lectin activity that defines their role in mucus.,Jarva MA, Lingford JP, John A, Soler NM, Scott NE, Goddard-Borger ED Nat Commun. 2020 May 13;11(1):2265. doi: 10.1038/s41467-020-16223-7. PMID:32404934[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chutipongtanate S, Nakagawa Y, Sritippayawan S, Pittayamateekul J, Parichatikanond P, Westley BR, May FE, Malasit P, Thongboonkerd V. Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor. J Clin Invest. 2005 Dec;115(12):3613-22. Epub 2005 Nov 23. PMID:16308573 doi:http://dx.doi.org/10.1172/JCI25342
- ↑ Jarva MA, Lingford JP, John A, Soler NM, Scott NE, Goddard-Borger ED. Trefoil factors share a lectin activity that defines their role in mucus. Nat Commun. 2020 May 13;11(1):2265. doi: 10.1038/s41467-020-16223-7. PMID:32404934 doi:http://dx.doi.org/10.1038/s41467-020-16223-7
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