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6vin

From Proteopedia

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Current revision

Crystallographic structure of the circularly permuted human Taspase1 protein

Structural highlights

6vin is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.04Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TASP1_HUMAN Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.

Publication Abstract from PubMed

Taspase1 is an Ntn-hydrolase overexpressed in primary human cancers, coordinating cancer cell proliferation, invasion, and metastasis. Loss of Taspase1 activity disrupts proliferation of human cancer cells in vitro and in mouse models of glioblastoma. Taspase1 is synthesized as an inactive proenzyme, becoming active upon intramolecular cleavage. The activation process changes the conformation of a long fragment at the C-terminus of the alpha subunit, for which no full-length structural information exists and whose function is poorly understood. We present a cloning strategy to generate a circularly permuted form of Taspase1 to determine the crystallographic structure of active Taspase1. We discovered that this region forms a long helix and is indispensable for the catalytic activity of Taspase1. Our study highlights the importance of this element for the enzymatic activity of Ntn-hydrolases, suggesting that it could be a potential target for the design of inhibitors with potential to be developed into anticancer therapeutics.

Structural insights into the function of the catalytically active human Taspase1.,Nagaratnam N, Delker SL, Jernigan R, Edwards TE, Snider J, Thifault D, Williams D, Nannenga BL, Stofega M, Sambucetti L, Hsieh JJ, Flint AJ, Fromme P, Martin-Garcia JM Structure. 2021 Mar 25. pii: S0969-2126(21)00081-2. doi:, 10.1016/j.str.2021.03.008. PMID:33784495^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nagaratnam N, Delker SL, Jernigan R, Edwards TE, Snider J, Thifault D, Williams D, Nannenga BL, Stofega M, Sambucetti L, Hsieh JJ, Flint AJ, Fromme P, Martin-Garcia JM. Structural insights into the function of the catalytically active human Taspase1. Structure. 2021 Mar 25. pii: S0969-2126(21)00081-2. doi:, 10.1016/j.str.2021.03.008. PMID:33784495 doi:http://dx.doi.org/10.1016/j.str.2021.03.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6vin"

Categories: Homo sapiens | Large Structures | Fromme P | Martin-Garcia JM

@@ Line 3: / Line 3: @@
 <StructureSection load='6vin' size='340' side='right'caption='[[6vin]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6vin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VIN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6vin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VIN FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TASP1, C20orf13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.04&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vin OCA], [https://pdbe.org/6vin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vin RCSB], [https://www.ebi.ac.uk/pdbsum/6vin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vin ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN]] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
+[https://www.uniprot.org/uniprot/TASP1_HUMAN TASP1_HUMAN] Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Fromme, P]]
+[[Category: Fromme P]]
-[[Category: Martin-Garcia, J M]]
+[[Category: Martin-Garcia JM]]
-[[Category: Asparaginase]]
-[[Category: Enzyme]]
-[[Category: Hydrolase]]
-[[Category: Taspase1]]
-[[Category: X-ray structure]]

6vin

From Proteopedia

Current revision

Crystallographic structure of the circularly permuted human Taspase1 protein

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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