1ngm

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[[Image:1ngm.gif|left|200px]]
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{{Structure
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{{STRUCTURE_1ngm| PDB=1ngm | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ngm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngm OCA], [http://www.ebi.ac.uk/pdbsum/1ngm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ngm RCSB]</span>
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'''Crystal structure of a yeast Brf1-TBP-DNA ternary complex'''
'''Crystal structure of a yeast Brf1-TBP-DNA ternary complex'''
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[[Category: Sigler, P B.]]
[[Category: Sigler, P B.]]
[[Category: Wang, J.]]
[[Category: Wang, J.]]
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[[Category: brf]]
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[[Category: Brf]]
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[[Category: brf1]]
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[[Category: Brf1]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: taf]]
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[[Category: Taf]]
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[[Category: tbp]]
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[[Category: Tbp]]
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[[Category: tfiiib]]
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[[Category: Tfiiib]]
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[[Category: transcription]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:30:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:30:08 2008''
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Revision as of 23:30, 2 May 2008

Image:1ngm.gif

Template:STRUCTURE 1ngm

Crystal structure of a yeast Brf1-TBP-DNA ternary complex


Overview

Transcription factor IIIB (TFIIIB), consisting of the TATA-binding protein (TBP), TFIIB-related factor (Brf1) and Bdp1, is a central component in basal and regulated transcription by RNA polymerase III. TFIIIB recruits its polymerase to the promoter and subsequently has an essential role in the formation of the open initiation complex. The amino-terminal half of Brf1 shares a high degree of sequence similarity with the polymerase II general transcription factor TFIIB, but it is the carboxy-terminal half of Brf1 that contributes most of its binding affinity with TBP. The principal anchoring region is located between residues 435 and 545 of yeast Brf1, comprising its homology domain II. The same region also provides the primary interface for assembling Bdp1 into the TFIIIB complex. We report here a 2.95 A resolution crystal structure of the ternary complex containing Brf1 homology domain II, the conserved region of TBP and 19 base pairs of U6 promoter DNA. The structure reveals the core interface for assembly of TFIIIB and demonstrates how the loosely packed Brf1 domain achieves remarkable binding specificity with the convex and lateral surfaces of TBP.

About this Structure

1NGM is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of a transcription factor IIIB core interface ternary complex., Juo ZS, Kassavetis GA, Wang J, Geiduschek EP, Sigler PB, Nature. 2003 Apr 3;422(6931):534-9. Epub 2003 Mar 26. PMID:12660736 Page seeded by OCA on Sat May 3 02:30:26 2008

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