1ngn
From Proteopedia
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'''Mismatch repair in methylated DNA. Structure of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4''' | '''Mismatch repair in methylated DNA. Structure of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4''' | ||
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[[Category: Wu, P.]] | [[Category: Wu, P.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:30:29 2008'' | |
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Revision as of 23:30, 2 May 2008
Mismatch repair in methylated DNA. Structure of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4
Overview
MBD4 is a member of the methyl-CpG-binding protein family. It contains two DNA binding domains, an amino-proximal methyl-CpG binding domain (MBD) and a C-terminal mismatch-specific glycosylase domain. Limited in vitro proteolysis of mouse MBD4 yields two stable fragments: a 139-residue fragment including the MBD, and the other 155-residue fragment including the glycosylase domain. Here we show that the latter fragment is active as a glycosylase on a DNA duplex containing a G:T mismatch within a CpG sequence context. The crystal structure confirmed the C-terminal domain is a member of the helix-hairpin-helix DNA glycosylase superfamily. The MBD4 active site is situated in a cleft that likely orients and binds DNA. Modeling studies suggest the mismatched target nucleotide will be flipped out into the active site where candidate residues for catalysis and substrate specificity are present.
About this Structure
1NGN is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Mismatch repair in methylated DNA. Structure and activity of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4., Wu P, Qiu C, Sohail A, Zhang X, Bhagwat AS, Cheng X, J Biol Chem. 2003 Feb 14;278(7):5285-91. Epub 2002 Nov 26. PMID:12456671 Page seeded by OCA on Sat May 3 02:30:29 2008
