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5ld5

From Proteopedia

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Crystal structure of a bacterial dehydrogenase at 2.19 Angstroms resolution

Structural highlights

5ld5 is a 4 chain structure with sequence from Fannyhessea vaginae DSM 15829. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1906Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

F1T6A5_9ACTO

Publication Abstract from PubMed

The Gram-positive anaerobic human pathogenic bacterium Atopobium vaginae causes most diagnosed cases of bacterial vaginosis as well as opportunistic infections in immunocompromised patients. In addition to its well-established role in carbohydrate metabolism, D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Streptococcus pyogenes and S. pneumoniae have been reported to act as extracellular virulence factors during streptococcal infections. Here, we report the crystal structure of GAPDH from A. vaginae (AvGAPDH) at 2.19 A resolution. The refined model has a crystallographic Rfree of 22.6%. AvGAPDH is a homotetramer wherein each subunit is bound to a nicotinamide adenine dinucleotide (NAD+) molecule. The AvGAPDH enzyme fulfills essential glycolytic as well as moonlight (non-glycolytic) functions, both of which might be targets of chemotherapeutic intervention. We report that AvGAPDH interacts in vitro with the human C5a anaphylatoxin and inhibits C5a-specific granulocyte chemotaxis, thereby suggesting the participation of AvGAPDH in complement-targeted immunoevasion in a context of infection. The availability of high-quality structures of AvGAPDH and other homologous virulence factors from Gram-positive pathogens is critical for drug discovery programs. In this study, sequence and structural differences between AvGAPDH and related bacterial and eukaryotic GAPDH enzymes are reported in an effort to understand how to subvert the immunoevasive properties of GAPDH and evaluate the potential of AvGAPDH as a druggable target.

Crystal Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Gram-Positive Bacterial Pathogen A. vaginae, an Immunoevasive Factor that Interacts with the Human C5a Anaphylatoxin.,Querol-Garcia J, Fernandez FJ, Marin AV, Gomez S, Fulla D, Melchor-Tafur C, Franco-Hidalgo V, Alberti S, Juanhuix J, Rodriguez de Cordoba S, Regueiro JR, Vega MC Front Microbiol. 2017 Apr 10;8:541. doi: 10.3389/fmicb.2017.00541. eCollection, 2017. PMID:28443070^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Querol-Garcia J, Fernandez FJ, Marin AV, Gomez S, Fulla D, Melchor-Tafur C, Franco-Hidalgo V, Alberti S, Juanhuix J, Rodriguez de Cordoba S, Regueiro JR, Vega MC. Crystal Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Gram-Positive Bacterial Pathogen A. vaginae, an Immunoevasive Factor that Interacts with the Human C5a Anaphylatoxin. Front Microbiol. 2017 Apr 10;8:541. doi: 10.3389/fmicb.2017.00541. eCollection, 2017. PMID:28443070 doi:http://dx.doi.org/10.3389/fmicb.2017.00541

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ld5"

@@ Line 3: / Line 3: @@
 <StructureSection load='5ld5' size='340' side='right'caption='[[5ld5]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ld5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LD5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LD5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ld5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fannyhessea_vaginae_DSM_15829 Fannyhessea vaginae DSM 15829]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LD5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1906&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ld5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ld5 OCA], [http://pdbe.org/5ld5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ld5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ld5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ld5 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ld5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ld5 OCA], [https://pdbe.org/5ld5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ld5 RCSB], [https://www.ebi.ac.uk/pdbsum/5ld5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ld5 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/F1T6A5_9ACTO F1T6A5_9ACTO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 26: @@
 __TOC__
 </StructureSection>
+[[Category: Fannyhessea vaginae DSM 15829]]
 [[Category: Large Structures]]
-[[Category: Fernandez, F J]]
+[[Category: Fernandez FJ]]
-[[Category: Fulla, D]]
+[[Category: Fulla D]]
-[[Category: Gomez, S]]
+[[Category: Gomez S]]
-[[Category: Juanhuix, J]]
+[[Category: Juanhuix J]]
-[[Category: Querol-Garcia, J]]
+[[Category: Querol-Garcia J]]
-[[Category: Vega, M C]]
+[[Category: Vega MC]]
-[[Category: Atopobium vaginae]]
-[[Category: Dehydrogenase]]
-[[Category: Gapdh]]
-[[Category: Nad]]
-[[Category: Oxidoreductase]]

5ld5

From Proteopedia

Current revision

Crystal structure of a bacterial dehydrogenase at 2.19 Angstroms resolution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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