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| | ==Crystal structure of the heterodimeric GEF Mon1-Ccz1 in complex with Ypt7== | | ==Crystal structure of the heterodimeric GEF Mon1-Ccz1 in complex with Ypt7== |
| - | <StructureSection load='5ldd' size='340' side='right' caption='[[5ldd]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='5ldd' size='340' side='right'caption='[[5ldd]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ldd]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LDD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LDD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ldd]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LDD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LDD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.496Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0067370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0050970 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0065990 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ldd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ldd OCA], [http://pdbe.org/5ldd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ldd RCSB], [http://www.ebi.ac.uk/pdbsum/5ldd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ldd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ldd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ldd OCA], [https://pdbe.org/5ldd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ldd RCSB], [https://www.ebi.ac.uk/pdbsum/5ldd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ldd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G0SGS3_CHATD G0SGS3_CHATD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Kiontke, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Kuemmel, D]] | + | [[Category: Kiontke S]] |
| - | [[Category: Endosomal maturation]] | + | [[Category: Kuemmel D]] |
| - | [[Category: Heterodimeric gef protein]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Rab gtpase]]
| + | |
| Structural highlights
Function
G0SGS3_CHATD
Publication Abstract from PubMed
The Mon1-Ccz1 complex (MC1) is the guanine nucleotide exchange factor (GEF) for the Rab GTPase Ypt7/Rab7 and is required for endosomal maturation and fusion at the vacuole/lysosome. Here we present the overall architecture of MC1 from Chaetomium thermophilum, and in combining biochemical studies and mutational analysis in yeast, we identify the domains required for catalytic activity, complex assembly and localization of MC1. The crystal structure of a catalytic MC1 core complex bound to Ypt7 provides mechanistic insight into its function. We pinpoint the determinants that allow for a discrimination of the Rab7-like Ypt7 over the Rab5-like Vps21, which are both located on the same membrane. MC1 shares structural similarities with the TRAPP complex, but employs a novel mechanism to promote nucleotide exchange that utilizes a conserved lysine residue of Ypt7, which is inserted upon MC1 binding into the nucleotide-binding pocket of Ypt7 and contributes to specificity.
Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1-Ccz1.,Kiontke S, Langemeyer L, Kuhlee A, Schuback S, Raunser S, Ungermann C, Kummel D Nat Commun. 2017 Jan 4;8:14034. doi: 10.1038/ncomms14034. PMID:28051187[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kiontke S, Langemeyer L, Kuhlee A, Schuback S, Raunser S, Ungermann C, Kummel D. Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1-Ccz1. Nat Commun. 2017 Jan 4;8:14034. doi: 10.1038/ncomms14034. PMID:28051187 doi:http://dx.doi.org/10.1038/ncomms14034
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