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| | ==A viral capsid:antibody complex== | | ==A viral capsid:antibody complex== |
| - | <StructureSection load='5ldn' size='340' side='right' caption='[[5ldn]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='5ldn' size='340' side='right'caption='[[5ldn]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ldn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ade05 Ade05] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LDN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ldn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_5 Human adenovirus 5] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LDN FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28285 ADE05])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ldn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ldn OCA], [http://pdbe.org/5ldn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ldn RCSB], [http://www.ebi.ac.uk/pdbsum/5ldn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ldn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ldn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ldn OCA], [https://pdbe.org/5ldn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ldn RCSB], [https://www.ebi.ac.uk/pdbsum/5ldn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ldn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CAPSH_ADE05 CAPSH_ADE05]] Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein (By similarity). Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus. | + | [https://www.uniprot.org/uniprot/CAPSH_ADE05 CAPSH_ADE05] Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein (By similarity). Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| | *[[Antibody 3D structures|Antibody 3D structures]] | | *[[Antibody 3D structures|Antibody 3D structures]] |
| | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | + | *[[3D structures of non-human antibody|3D structures of non-human antibody]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ade05]] | + | [[Category: Human adenovirus 5]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: James, L]] | + | [[Category: Mus musculus]] |
| - | [[Category: Adenovirus]] | + | [[Category: James L]] |
| - | [[Category: Neutralization]]
| + | |
| - | [[Category: Trim21]]
| + | |
| - | [[Category: Viral protein]]
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| Structural highlights
Function
CAPSH_ADE05 Major capsid protein that self-associates to form 240 hexon trimers, each in the shape of a hexagon, building most of the pseudo T=25 capsid. Assembled into trimeric units with the help of the chaperone shutoff protein (By similarity). Transported by pre-protein VI to the nucleus where it associates with other structural proteins to form an empty capsid. Might be involved, through its interaction with host dyneins, in the intracellular microtubule-dependent transport of incoming viral capsid to the nucleus.
Publication Abstract from PubMed
During infection with non-enveloped viruses, antibodies stimulate immunity from inside cells by activating the cytosolic Fc receptor TRIM21. This intracellular humoral response relies on opsonized viral particles reaching the cytosol intact but the antigenic and kinetic constraints involved are unknown. We have solved the structure of a potent TRIM21-dependent neutralizing antibody in complex with human adenovirus 5 hexon and show how these properties influence immune activity. Structure-guided mutagenesis was used to generate antibodies with 20,000-fold variation in affinity, on-rates that differ by ~50-fold and off-rates by >175-fold. Characterization of these variants during infection revealed that TRIM21-dependent neutralization and NFkappaB activation was largely unaffected by on-rate kinetics. In contrast, TRIM21 antiviral activity was exquisitely dependent upon off-rate, with sub-muM affinity antibodies nevertheless unable to stimulate signaling because of fast dissociation kinetics. These results define the antibody properties required to elicit an efficient intracellular immune response during viral infection.
Antibody-antigen kinetics constrain intracellular humoral immunity.,Bottermann M, Lode HE, Watkinson RE, Foss S, Sandlie I, Andersen JT, James LC Sci Rep. 2016 Nov 24;6:37457. doi: 10.1038/srep37457. PMID:27881870[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Bottermann M, Lode HE, Watkinson RE, Foss S, Sandlie I, Andersen JT, James LC. Antibody-antigen kinetics constrain intracellular humoral immunity. Sci Rep. 2016 Nov 24;6:37457. doi: 10.1038/srep37457. PMID:27881870 doi:http://dx.doi.org/10.1038/srep37457
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