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| | <StructureSection load='5lfg' size='340' side='right'caption='[[5lfg]], [[Resolution|resolution]] 1.94Å' scene=''> | | <StructureSection load='5lfg' size='340' side='right'caption='[[5lfg]], [[Resolution|resolution]] 1.94Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lfg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trematomus_newnesi Trematomus newnesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LFG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LFG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lfg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trematomus_newnesi Trematomus newnesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LFG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1t1n|1t1n]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lfg OCA], [https://pdbe.org/5lfg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lfg RCSB], [https://www.ebi.ac.uk/pdbsum/5lfg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lfg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lfg OCA], [http://pdbe.org/5lfg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lfg RCSB], [http://www.ebi.ac.uk/pdbsum/5lfg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lfg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HBB_TRENE HBB_TRENE]] Involved in oxygen transport from gills to the various peripheral tissues. | + | [https://www.uniprot.org/uniprot/HBA1_TRENE HBA1_TRENE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Trematomus newnesi]] | | [[Category: Trematomus newnesi]] |
| - | [[Category: Mazzarella, L]] | + | [[Category: Mazzarella L]] |
| - | [[Category: Merlino, A]] | + | [[Category: Merlino A]] |
| - | [[Category: Vergara, A]] | + | [[Category: Vergara A]] |
| - | [[Category: Vitagliano, L]] | + | [[Category: Vitagliano L]] |
| - | [[Category: Globin fold]]
| + | |
| - | [[Category: Oxygen transport]]
| + | |
| Structural highlights
Function
HBA1_TRENE
Publication Abstract from PubMed
Although the end points of the functional transitions of tetrameric hemoglobins (Hbs) have been well characterized, atomic-resolution data on R-T intermediate states are extremely limited. Herein, the X-ray structures of two independent tetramers of the fully ligated carbomonoxy form of Trematomus newnesi hemoglobin (Hb1Tn) within the same crystal are described. These structures show peculiar features in the heme pocket, EF corner, and tertiary/quaternary structure. Distal histidine side chains have a propensity to swing out of the heme pocket and thus allow compression of the EF corner. In this rotameric state, the distal His group does not interact with the CO ligand, consistent with FTIR spectra recorded in solution. At the quaternary-structure level, one tetramer is an intermediate R-T state, whereas the other assumes a T-like structure. Altogether, the structures of these tetramers provide the best available atomic-level picture of the R-->T transition of vertebrate Hbs.
Fine Sampling of the R-->T Quaternary-Structure Transition of a Tetrameric Hemoglobin.,Vitagliano L, Mazzarella L, Merlino A, Vergara A Chemistry. 2017 Jan 12;23(3):605-613. doi: 10.1002/chem.201603421. Epub 2016 Dec , 7. PMID:27808442[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Vitagliano L, Mazzarella L, Merlino A, Vergara A. Fine Sampling of the R-->T Quaternary-Structure Transition of a Tetrameric Hemoglobin. Chemistry. 2017 Jan 12;23(3):605-613. doi: 10.1002/chem.201603421. Epub 2016 Dec , 7. PMID:27808442 doi:http://dx.doi.org/10.1002/chem.201603421
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