1nhl

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[[Image:1nhl.jpg|left|200px]]
[[Image:1nhl.jpg|left|200px]]
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{{Structure
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|PDB= 1nhl |SIZE=350|CAPTION= <scene name='initialview01'>1nhl</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1nhl", creates the "Structure Box" on the page.
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|SITE=
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|GENE= SNAP23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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{{STRUCTURE_1nhl| PDB=1nhl | SCENE= }}
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|RELATEDENTRY=[[1sfc|1SFC]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhl OCA], [http://www.ebi.ac.uk/pdbsum/1nhl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nhl RCSB]</span>
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'''SNAP-23N Structure'''
'''SNAP-23N Structure'''
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[[Category: Song, H K.]]
[[Category: Song, H K.]]
[[Category: Xu, Y.]]
[[Category: Xu, Y.]]
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[[Category: coiled-coil]]
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[[Category: Coiled-coil]]
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[[Category: snare]]
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[[Category: Snare]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:32:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:30:30 2008''
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Revision as of 23:32, 2 May 2008

Image:1nhl.jpg

Template:STRUCTURE 1nhl

SNAP-23N Structure


Overview

SNARE proteins mediate intracellular membrane fusion by forming a coiled-coil complex to merge opposing membranes. A "fusion-active" neuronal SNARE complex is a parallel four-helix bundle containing two coiled-coil domains from SNAP-25 and one coiled-coil domain each from syntaxin-1a and VAMP-2. "Prefusion" assembly intermediate complexes can also form from these SNAREs. We studied the N-terminal coiled-coil domain of SNAP-23 (SNAP-23N), a non-neuronal homologue of SNAP-25, and its interaction with other coiled-coil domains. SNAP-23N can assemble spontaneously with the coiled-coil domains from SNAP-23C, syntaxin-4, and VAMP-3 to form a heterotetrameric complex. Unexpectedly, pure SNAP-23N crystallizes as a coiled-coil homotetrameric complex. The four helices have a parallel orientation and are symmetrical about the long axis. The complex is stabilized through the interaction of conserved hydrophobic residues comprising the a and d positions of the coiled-coil heptad repeats. In addition, a central, highly conserved glutamine residue (Gln-48) is buried within the interface by hydrogen bonding between glutamine side chains derived from adjacent subunits and to solvent molecules. A comparison of the SNAP-23N structure to other SNARE complex structures reveals how a simple coiled-coil motif can form diverse SNARE complexes.

About this Structure

1NHL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:12556468 Page seeded by OCA on Sat May 3 02:32:25 2008

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