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| | <StructureSection load='6w1b' size='340' side='right'caption='[[6w1b]], [[Resolution|resolution]] 2.31Å' scene=''> | | <StructureSection load='6w1b' size='340' side='right'caption='[[6w1b]], [[Resolution|resolution]] 2.31Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6w1b]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W1B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W1B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6w1b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W1B FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEE:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>PEE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sftpb, Sftp3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEE:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>PEE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w1b OCA], [http://pdbe.org/6w1b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w1b RCSB], [http://www.ebi.ac.uk/pdbsum/6w1b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w1b ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w1b OCA], [https://pdbe.org/6w1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w1b RCSB], [https://www.ebi.ac.uk/pdbsum/6w1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w1b ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PSPB_MOUSE PSPB_MOUSE]] Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. | + | [https://www.uniprot.org/uniprot/PSPB_MOUSE PSPB_MOUSE] Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Bodnar, N O]] | + | [[Category: Bodnar NO]] |
| - | [[Category: Rapoport, T A]] | + | [[Category: Rapoport TA]] |
| - | [[Category: Lipid transfer]]
| + | |
| - | [[Category: Lipid-binding]]
| + | |
| - | [[Category: Saposin-like]]
| + | |
| - | [[Category: Surfactant protein]]
| + | |
| Structural highlights
Function
PSPB_MOUSE Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Publication Abstract from PubMed
Breathing depends on pulmonary surfactant, a mixture of phospholipids and proteins, secreted by alveolar type II cells. Surfactant requires lamellar bodies (LBs), organelles containing densely packed concentric membrane layers, for storage and secretion. LB biogenesis remains mysterious but requires surfactant protein B (SP-B), which is synthesized as a precursor (pre-proSP-B) that is cleaved during trafficking into three related proteins. Here, we elucidate the functions and cooperation of these proteins in LB formation. We show that the N-terminal domain of proSP-B is a phospholipid-binding and -transfer protein whose activities are required for proSP-B export from the endoplasmic reticulum (ER) and sorting to LBs, the conversion of proSP-B into lipoprotein particles, and neonatal viability in mice. The C-terminal domain facilitates ER export of proSP-B. The mature middle domain, generated after proteolytic cleavage of proSP-B, generates the striking membrane layers characteristic of LBs. Together, our results lead to a mechanistic model of LB biogenesis.
Mechanism of Lamellar Body Formation by Lung Surfactant Protein B.,Sever N, Milicic G, Bodnar NO, Wu X, Rapoport TA Mol Cell. 2020 Nov 17. pii: S1097-2765(20)30776-0. doi:, 10.1016/j.molcel.2020.10.042. PMID:33242393[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sever N, Milicic G, Bodnar NO, Wu X, Rapoport TA. Mechanism of Lamellar Body Formation by Lung Surfactant Protein B. Mol Cell. 2020 Nov 17. pii: S1097-2765(20)30776-0. doi:, 10.1016/j.molcel.2020.10.042. PMID:33242393 doi:http://dx.doi.org/10.1016/j.molcel.2020.10.042
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