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| | <StructureSection load='6w5q' size='340' side='right'caption='[[6w5q]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='6w5q' size='340' side='right'caption='[[6w5q]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6w5q]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W5Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W5Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6w5q]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W5Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA4699 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w5q OCA], [http://pdbe.org/6w5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w5q RCSB], [http://www.ebi.ac.uk/pdbsum/6w5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w5q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w5q OCA], [https://pdbe.org/6w5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w5q RCSB], [https://www.ebi.ac.uk/pdbsum/6w5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w5q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HV97_PSEAE Q9HV97_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Caveney, N A]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Robb, C S]] | + | [[Category: Caveney NA]] |
| - | [[Category: Simorre, J P]] | + | [[Category: Robb CS]] |
| - | [[Category: Strynadka, N C.J]] | + | [[Category: Simorre JP]] |
| - | [[Category: Activator]] | + | [[Category: Strynadka NCJ]] |
| - | [[Category: Cell wall]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Function
Q9HV97_PSEAE
Publication Abstract from PubMed
Peptidoglycan (PG) is an essential component of the bacterial cell wall and is assembled from a lipid II precursor by glycosyltransferase and transpeptidase reactions catalyzed in particular by bifunctional class A penicillin-binding proteins (aPBPs). In the major clinical pathogen Pseudomonas aeruginosa, PBP1B is anchored within the cytoplasmic membrane but regulated by a bespoke outer membrane-localized lipoprotein known as LpoP. Here, we report the structure of LpoP, showing an extended N-terminal, flexible tether followed by a well-ordered C-terminal tandem-tetratricopeptide repeat domain. We show that LpoP stimulates both PBP1B transpeptidase and glycosyltransferase activities in vitro and interacts directly via its C terminus globular domain with the central UB2H domain of PBP1B. Contrary to the situation in E. coli, P. aeruginosa CpoB does not regulate PBP1B/LpoP in vitro. We propose a mechanism that helps to underscore similarities and differences in class A PBP activation across Gram-negative bacteria.
Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa.,Caveney NA, Egan AJF, Ayala I, Laguri C, Robb CS, Breukink E, Vollmer W, Strynadka NCJ, Simorre JP Structure. 2020 Apr 9. pii: S0969-2126(20)30094-0. doi:, 10.1016/j.str.2020.03.012. PMID:32320673[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Caveney NA, Egan AJF, Ayala I, Laguri C, Robb CS, Breukink E, Vollmer W, Strynadka NCJ, Simorre JP. Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa. Structure. 2020 Apr 9. pii: S0969-2126(20)30094-0. doi:, 10.1016/j.str.2020.03.012. PMID:32320673 doi:http://dx.doi.org/10.1016/j.str.2020.03.012
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