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| | <StructureSection load='6wpi' size='340' side='right'caption='[[6wpi]], [[Resolution|resolution]] 3.02Å' scene=''> | | <StructureSection load='6wpi' size='340' side='right'caption='[[6wpi]], [[Resolution|resolution]] 3.02Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6wpi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WPI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wpi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TCE:3,3,3-PHOSPHANETRIYLTRIPROPANOIC+ACID'>TCE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.02Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NOP9, YJL010C, J1357 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TCE:3,3,3-PHOSPHANETRIYLTRIPROPANOIC+ACID'>TCE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpi OCA], [http://pdbe.org/6wpi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wpi RCSB], [http://www.ebi.ac.uk/pdbsum/6wpi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpi ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpi OCA], [https://pdbe.org/6wpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpi RCSB], [https://www.ebi.ac.uk/pdbsum/6wpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpi ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST]] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref> | + | [https://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hall, T M.T]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Qiu, C]] | + | [[Category: Hall TMT]] |
| - | [[Category: Zhang, J]] | + | [[Category: Qiu C]] |
| - | [[Category: Nop9]] | + | [[Category: Zhang J]] |
| - | [[Category: Puf protein]]
| + | |
| - | [[Category: Ribosome biogenesis]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
| Structural highlights
Function
NOP9_YEAST RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.[1] [2]
Publication Abstract from PubMed
Nop9 is an essential factor in the processing of pre-ribosomal RNA. Its absence in yeast is lethal, and defects in the human ortholog are associated with breast cancer, autoimmunity, and learning/language impairment. PUF family RNA-binding proteins are best known for sequence-specific RNA recognition, and most contain eight alpha-helical repeats that bind to the RNA bases of single-stranded RNA. Nop9 is an unusual member of this family in that it contains eleven repeats and recognizes both RNA structure and sequence. Here we report a crystal structure of Saccharomyces cerevisiae Nop9 in complex with its target RNA within the 20S pre-ribosomal RNA. This structure reveals that Nop9 brings together a C-terminal module recognizing the 5' single-stranded region of the RNA and a bifunctional N-terminal module recognizing the central double-stranded stem region. We further show that the 3' single-stranded region of the 20S target RNA adds sequence-independent binding energy to the RNA-Nop9 interaction. Both the N- and C-terminal modules retain the characteristic sequence-specific recognition of PUF proteins but the N-terminal module has also evolved a distinct interface, which allows Nop9 to recognize either single-stranded RNA sequences or RNAs with a combination of single-stranded and structured elements.
Nop9 recognizes structured and single-stranded RNA elements of pre-ribosomal RNA.,Zhang J, Teramoto T, Qiu C, Wine RN, Gonzalez LE, Baserga SJ, Hall TM RNA. 2020 May 5. pii: rna.075416.120. doi: 10.1261/rna.075416.120. PMID:32371454[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wade CH, Umbarger MA, McAlear MA. The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes. Yeast. 2006 Mar;23(4):293-306. PMID:16544271 doi:http://dx.doi.org/10.1002/yea.1353
- ↑ Thomson E, Rappsilber J, Tollervey D. Nop9 is an RNA binding protein present in pre-40S ribosomes and required for 18S rRNA synthesis in yeast. RNA. 2007 Dec;13(12):2165-74. Epub 2007 Oct 23. PMID:17956976 doi:http://dx.doi.org/10.1261/rna.747607
- ↑ Zhang J, Teramoto T, Qiu C, Wine RN, Gonzalez LE, Baserga SJ, Hall TM. Nop9 recognizes structured and single-stranded RNA elements of pre-ribosomal RNA. RNA. 2020 May 5. pii: rna.075416.120. doi: 10.1261/rna.075416.120. PMID:32371454 doi:http://dx.doi.org/10.1261/rna.075416.120
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