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6ws6

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Structural and functional analysis of a potent sarbecovirus neutralizing antibody

Structural highlights

6ws6 is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a newly emerged coronavirus that is responsible for the current pandemic of coronavirus disease 2019 (COVID-19), which has resulted in more than 3.7 million infections and 260,000 deaths as of 6 May 2020(1,2). Vaccine and therapeutic discovery efforts are paramount to curb the pandemic spread of this zoonotic virus. The SARS-CoV-2 spike (S) glycoprotein promotes entry into host cells and is the main target of neutralizing antibodies. Here we describe several monoclonal antibodies that target the S glycoprotein of SARS-CoV-2, which we identified from memory B cells of an individual who was infected with severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003. One antibody (named S309) potently neutralizes SARS-CoV-2 and SARS-CoV pseudoviruses as well as authentic SARS-CoV-2, by engaging the receptor-binding domain of the S glycoprotein. Using cryo-electron microscopy and binding assays, we show that S309 recognizes an epitope containing a glycan that is conserved within the Sarbecovirus subgenus, without competing with receptor attachment. Antibody cocktails that include S309 in combination with other antibodies that we identified further enhanced SARS-CoV-2 neutralization, and may limit the emergence of neutralization-escape mutants. These results pave the way for using S309 and antibody cocktails containing S309 for prophylaxis in individuals at a high risk of exposure or as a post-exposure therapy to limit or treat severe disease.

Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody.,Pinto D, Park YJ, Beltramello M, Walls AC, Tortorici MA, Bianchi S, Jaconi S, Culap K, Zatta F, De Marco A, Peter A, Guarino B, Spreafico R, Cameroni E, Case JB, Chen RE, Havenar-Daughton C, Snell G, Telenti A, Virgin HW, Lanzavecchia A, Diamond MS, Fink K, Veesler D, Corti D Nature. 2020 Jul;583(7815):290-295. doi: 10.1038/s41586-020-2349-y. Epub 2020 May, 18. PMID:32422645^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pinto D, Park YJ, Beltramello M, Walls AC, Tortorici MA, Bianchi S, Jaconi S, Culap K, Zatta F, De Marco A, Peter A, Guarino B, Spreafico R, Cameroni E, Case JB, Chen RE, Havenar-Daughton C, Snell G, Telenti A, Virgin HW, Lanzavecchia A, Diamond MS, Fink K, Veesler D, Corti D. Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody. Nature. 2020 Jul;583(7815):290-295. doi: 10.1038/s41586-020-2349-y. Epub 2020 May, 18. PMID:32422645 doi:http://dx.doi.org/10.1038/s41586-020-2349-y

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ws6"

@@ Line 3: / Line 3: @@
 <StructureSection load='6ws6' size='340' side='right'caption='[[6ws6]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ws6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WS6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ws6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WS6 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JEF:O-(O-(2-AMINOPROPYL)-O-(2-METHOXYETHYL)POLYPROPYLENE+GLYCOL+500)'>JEF</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JEF:O-(O-(2-AMINOPROPYL)-O-(2-METHOXYETHYL)POLYPROPYLENE+GLYCOL+500)'>JEF</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ws6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ws6 OCA], [https://pdbe.org/6ws6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ws6 RCSB], [https://www.ebi.ac.uk/pdbsum/6ws6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ws6 ProSAT]</span></td></tr>
 </table>
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Beltramello, M]]
+[[Category: Beltramello M]]
-[[Category: Bianchi, S]]
+[[Category: Bianchi S]]
-[[Category: Cameroni, E]]
+[[Category: Cameroni E]]
-[[Category: Case, J B]]
+[[Category: Case JB]]
-[[Category: Chen, R E]]
+[[Category: Chen RE]]
-[[Category: Corti, D]]
+[[Category: Corti D]]
-[[Category: Culap, K]]
+[[Category: Culap K]]
-[[Category: Diamond, M S]]
+[[Category: Diamond MS]]
-[[Category: Fink, K]]
+[[Category: Fink K]]
-[[Category: Guarino, B]]
+[[Category: Guarino B]]
-[[Category: Havenar-Daughton, C]]
+[[Category: Havenar-Daughton C]]
-[[Category: Jaconi, S]]
+[[Category: Jaconi S]]
-[[Category: Lanzavecchia, A]]
+[[Category: Lanzavecchia A]]
-[[Category: Marco, A D]]
+[[Category: Marco AD]]
-[[Category: Park, Y J]]
+[[Category: Park YJ]]
-[[Category: Peter, A]]
+[[Category: Peter A]]
-[[Category: Pinto, D]]
+[[Category: Pinto D]]
-[[Category: Structural genomic]]
+[[Category: Snell G]]
-[[Category: Snell, G]]
+[[Category: Spreafico R]]
-[[Category: Spreafico, R]]
+[[Category: Telenti A]]
-[[Category: Telenti, A]]
+[[Category: Tortorici MA]]
-[[Category: Tortorici, M A]]
+[[Category: Veesler D]]
-[[Category: Veesler, D]]
+[[Category: Virgin HW]]
-[[Category: Virgin, H W]]
+[[Category: Walls AC]]
-[[Category: Walls, A C]]
+[[Category: Zatta F]]
-[[Category: Zatta, F]]
-[[Category: Fusion protein]]
-[[Category: Immune system]]
-[[Category: Neutralizing antibody]]
-[[Category: Sarbecovirus]]
-[[Category: Ssgcid]]

6ws6

From Proteopedia

Current revision

Structural and functional analysis of a potent sarbecovirus neutralizing antibody

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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