1nj3
From Proteopedia
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'''Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4''' | '''Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4''' | ||
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[[Category: Sundquist, W I.]] | [[Category: Sundquist, W I.]] | ||
[[Category: Wang, B.]] | [[Category: Wang, B.]] | ||
| - | [[Category: | + | [[Category: Beta-ribbon]] |
| - | [[Category: | + | [[Category: Npl4]] |
| - | [[Category: | + | [[Category: Nzf domain]] |
| - | [[Category: | + | [[Category: Rubredoxin knuckle]] |
| - | [[Category: | + | [[Category: Ubiquitin]] |
| - | [[Category: | + | [[Category: Zinc-finger]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:35:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:35, 2 May 2008
Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4
Overview
Ubiquitylated proteins are directed into a large number of different cellular pathways through interactions with effector proteins that contain conserved ubiquitin binding motifs. Here, we report the solution structure and ubiquitin binding properties of one such motif, the Npl4 zinc finger or RanBP2/Nup358 zinc finger (NZF) domain. Npl4 NZF forms a compact module composed of four antiparallel beta-strands linked by three ordered loops. A single zinc ion is coordinated by four conserved cysteines from the first and third loops, which form two rubredoxin knuckles. Npl4 NZF binds specifically, but weakly, to free ubiquitin using a conserved 13TF14 dipeptide to interact with the "Ile-44" surface of ubiquitin. Our studies reveal the structure of this versatile class of protein binding domains and provide a means for identifying the subset of NZF domains likely to bind ubiquitin.
About this Structure
1NJ3 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4., Wang B, Alam SL, Meyer HH, Payne M, Stemmler TL, Davis DR, Sundquist WI, J Biol Chem. 2003 May 30;278(22):20225-34. Epub 2003 Mar 18. PMID:12644454 Page seeded by OCA on Sat May 3 02:35:39 2008
