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| | <StructureSection load='6xph' size='340' side='right'caption='[[6xph]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6xph' size='340' side='right'caption='[[6xph]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6xph]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_intermedius_atcc_27335 Streptococcus intermedius atcc 27335]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XPH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XPH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xph]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_intermedius_SK54_=_ATCC_27335 Streptococcus intermedius SK54 = ATCC 27335]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XPH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6xpi|6xpi]], [[6xpj|6xpj]], [[6xpk|6xpk]], [[6xpl|6xpl]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HMPREF1654_00416 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1095731 Streptococcus intermedius ATCC 27335])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xph OCA], [https://pdbe.org/6xph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xph RCSB], [https://www.ebi.ac.uk/pdbsum/6xph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xph ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xph OCA], [http://pdbe.org/6xph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xph RCSB], [http://www.ebi.ac.uk/pdbsum/6xph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xph ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CUTR_STRIS CUTR_STRIS] A minor shell protein of the choline degradation-specific bacterial microcompartment (BMC). Proteins such as this one with circularly permuted BMC domains may play a key role in conferring heterogeneity and flexibility in this BMC.<ref>PMID:32885887</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Streptococcus intermedius atcc 27335]] | + | [[Category: Streptococcus intermedius SK54 = ATCC 27335]] |
| - | [[Category: Duilio, C]] | + | [[Category: Duilio C]] |
| - | [[Category: Nguyen, V N]] | + | [[Category: Nguyen VN]] |
| - | [[Category: Nie, M]] | + | [[Category: Nie M]] |
| - | [[Category: Ochoa, J M]] | + | [[Category: Ochoa JM]] |
| - | [[Category: Sawaya, M R]] | + | [[Category: Sawaya MR]] |
| - | [[Category: Yeates, T O]] | + | [[Category: Yeates TO]] |
| - | [[Category: Bmc]]
| + | |
| - | [[Category: Mcp]]
| + | |
| - | [[Category: Microcompartment]]
| + | |
| - | [[Category: Shell protein]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
CUTR_STRIS A minor shell protein of the choline degradation-specific bacterial microcompartment (BMC). Proteins such as this one with circularly permuted BMC domains may play a key role in conferring heterogeneity and flexibility in this BMC.[1]
Publication Abstract from PubMed
Bacterial microcompartments are protein-based organelles that carry out specialized metabolic functions in diverse bacteria. Their outer shells are built from several thousand protein subunits. Some of the architectural principles of bacterial microcompartments have been articulated, with lateral packing of flat hexameric BMC proteins providing the basic foundation for assembly. Nonetheless, a complete understanding has been elusive, partly owing to polymorphic mechanisms of assembly exhibited by most microcompartment types. An earlier study of one homologous BMC shell protein subfamily, EutS/PduU, revealed a profoundly bent, rather than flat, hexameric structure. The possibility of a specialized architectural role was hypothesized, but artifactual effects of crystallization could not be ruled out. Here we report a series of crystal structures of an orthologous protein, CutR, from a glycyl-radical type choline-utilizing microcompartment from the bacterium Streptococcus intermedius. Depending on crystal form, expression construct, and minor mutations, a range of novel quaternary architectures was observed, including two spiral hexagonal assemblies. A new graphical approach helps illuminate the variations in BMC hexameric structure, with results substantiating the idea that the EutS/PduU/CutR subfamily of BMC proteins may endow microcompartment shells with flexible modes of assembly.
Symmetry breaking and structural polymorphism in a bacterial microcompartment shell protein for choline utilization.,Ochoa JM, Nguyen VN, Nie M, Sawaya MR, Bobik TA, Yeates TO Protein Sci. 2020 Sep 4. doi: 10.1002/pro.3941. PMID:32885887[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ochoa JM, Nguyen VN, Nie M, Sawaya MR, Bobik TA, Yeates TO. Symmetry breaking and structural polymorphism in a bacterial microcompartment shell protein for choline utilization. Protein Sci. 2020 Sep 4. doi: 10.1002/pro.3941. PMID:32885887 doi:http://dx.doi.org/10.1002/pro.3941
- ↑ Ochoa JM, Nguyen VN, Nie M, Sawaya MR, Bobik TA, Yeates TO. Symmetry breaking and structural polymorphism in a bacterial microcompartment shell protein for choline utilization. Protein Sci. 2020 Sep 4. doi: 10.1002/pro.3941. PMID:32885887 doi:http://dx.doi.org/10.1002/pro.3941
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