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| | <StructureSection load='7jl4' size='340' side='right'caption='[[7jl4]], [[Resolution|resolution]] 1.92Å' scene=''> | | <StructureSection load='7jl4' size='340' side='right'caption='[[7jl4]], [[Resolution|resolution]] 1.92Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7jl4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JL4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7JL4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7jl4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JL4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TRIM65 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7jl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jl4 OCA], [http://pdbe.org/7jl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7jl4 RCSB], [http://www.ebi.ac.uk/pdbsum/7jl4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7jl4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jl4 OCA], [https://pdbe.org/7jl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jl4 RCSB], [https://www.ebi.ac.uk/pdbsum/7jl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jl4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TRI65_HUMAN TRI65_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ahmad, S]] | + | [[Category: Ahmad S]] |
| - | [[Category: Hur, S]] | + | [[Category: Hur S]] |
| - | [[Category: Kato, K]] | + | [[Category: Kato K]] |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Rig-i-like helicase]]
| + | |
| - | [[Category: Ubiquitin e3 ligase]]
| + | |
| Structural highlights
Function
TRI65_HUMAN
Publication Abstract from PubMed
RNA helicases and E3 ubiquitin ligases mediate many critical functions in cells, but their actions have largely been studied in distinct biological contexts. Here, we uncover evolutionarily conserved rules of engagement between RNA helicases and tripartite motif (TRIM) E3 ligases that lead to their functional coordination in vertebrate innate immunity. Using cryoelectron microscopy and biochemistry, we show that RIG-I-like receptors (RLRs), viral RNA receptors with helicase domains, interact with their cognate TRIM/TRIM-like E3 ligases through similar epitopes in the helicase domains. Their interactions are avidity driven, restricting the actions of TRIM/TRIM-like proteins and consequent immune activation to RLR multimers. Mass spectrometry and phylogeny-guided biochemical analyses further reveal that similar rules of engagement may apply to diverse RNA helicases and TRIM/TRIM-like proteins. Our analyses suggest not only conserved substrates for TRIM proteins but also, unexpectedly, deep evolutionary connections between TRIM proteins and RNA helicases, linking ubiquitin and RNA biology throughout animal evolution.
Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases.,Kato K, Ahmad S, Zhu Z, Young JM, Mu X, Park S, Malik HS, Hur S Mol Cell. 2020 Dec 16. pii: S1097-2765(20)30889-3. doi:, 10.1016/j.molcel.2020.11.047. PMID:33373584[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kato K, Ahmad S, Zhu Z, Young JM, Mu X, Park S, Malik HS, Hur S. Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases. Mol Cell. 2020 Dec 16. pii: S1097-2765(20)30889-3. doi:, 10.1016/j.molcel.2020.11.047. PMID:33373584 doi:http://dx.doi.org/10.1016/j.molcel.2020.11.047
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