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| | <StructureSection load='7jzt' size='340' side='right'caption='[[7jzt]], [[Resolution|resolution]] 3.77Å' scene=''> | | <StructureSection load='7jzt' size='340' side='right'caption='[[7jzt]], [[Resolution|resolution]] 3.77Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7jzt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebov-may Ebov-may]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7JZT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7jzt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JZT FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOV-May])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.77Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7jzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jzt OCA], [http://pdbe.org/7jzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7jzt RCSB], [http://www.ebi.ac.uk/pdbsum/7jzt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7jzt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jzt OCA], [https://pdbe.org/7jzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jzt RCSB], [https://www.ebi.ac.uk/pdbsum/7jzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jzt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VP40_EBOZM VP40_EBOZM]] Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.<ref>PMID:15892969</ref> <ref>PMID:16719918</ref> | + | [https://www.uniprot.org/uniprot/VP40_EBOZM VP40_EBOZM] Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.<ref>PMID:15892969</ref> <ref>PMID:16719918</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ebov-may]] | + | [[Category: Ebola virus - Mayinga, Zaire, 1976]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bornholdt, Z A]] | + | [[Category: Bornholdt ZA]] |
| - | [[Category: Norris, M J]] | + | [[Category: Norris MJ]] |
| - | [[Category: Saphire, E O]] | + | [[Category: Saphire EO]] |
| - | [[Category: Ebola virus]]
| + | |
| - | [[Category: Matrix protein]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| - | [[Category: Vp40]]
| + | |
| Structural highlights
Function
VP40_EBOZM Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.[1] [2]
Publication Abstract from PubMed
Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.
Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers.,Wan W, Clarke M, Norris MJ, Kolesnikova L, Koehler A, Bornholdt ZA, Becker S, Saphire EO, Briggs JA Elife. 2020 Oct 5;9. pii: 59225. doi: 10.7554/eLife.59225. PMID:33016878[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Irie T, Licata JM, Harty RN. Functional characterization of Ebola virus L-domains using VSV recombinants. Virology. 2005 Jun 5;336(2):291-8. PMID:15892969 doi:10.1016/j.virol.2005.03.027
- ↑ Johnson RF, Bell P, Harty RN. Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology. Virol J. 2006 May 23;3:31. PMID:16719918 doi:10.1186/1743-422X-3-31
- ↑ Wan W, Clarke M, Norris MJ, Kolesnikova L, Koehler A, Bornholdt ZA, Becker S, Saphire EO, Briggs JA. Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers. Elife. 2020 Oct 5;9. pii: 59225. doi: 10.7554/eLife.59225. PMID:33016878 doi:http://dx.doi.org/10.7554/eLife.59225
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