|
|
| Line 3: |
Line 3: |
| | <StructureSection load='7k16' size='340' side='right'caption='[[7k16]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='7k16' size='340' side='right'caption='[[7k16]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7k16]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K16 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7K16 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7k16]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tamana_bat_virus Tamana bat virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K16 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K16 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7k16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k16 OCA], [http://pdbe.org/7k16 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7k16 RCSB], [http://www.ebi.ac.uk/pdbsum/7k16 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7k16 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k16 OCA], [https://pdbe.org/7k16 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k16 RCSB], [https://www.ebi.ac.uk/pdbsum/7k16 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k16 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| Line 22: |
Line 22: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jones, R A]] | |
| - | [[Category: Kieft, J S]] | |
| - | [[Category: Exoribonuclease-resitant]] | |
| - | [[Category: Flavivirus]] | |
| - | [[Category: Ncrna]] | |
| - | [[Category: Rna]] | |
| | [[Category: Tamana bat virus]] | | [[Category: Tamana bat virus]] |
| - | [[Category: Xrn1]] | + | [[Category: Jones RA]] |
| - | [[Category: Xrrna]] | + | [[Category: Kieft JS]] |
| Structural highlights
Publication Abstract from PubMed
During infection by a flavivirus (FV), cells accumulate noncoding subgenomic flavivirus RNAs (sfRNAs) that interfere with several antiviral pathways. These sfRNAs are formed by structured RNA elements in the 3' untranslated region (UTR) of the viral genomic RNA, which block the progression of host cell exoribonucleases that have targeted the viral RNA. Previous work on these exoribonuclease-resistant RNAs (xrRNAs) from mosquito-borne FVs revealed a specific 3-dimensional fold with a unique topology in which a ring-like structure protectively encircles the 5' end of the xrRNA. Conserved nucleotides make specific tertiary interactions that support this fold. Examination of more divergent FVs reveals differences in their 3' UTR sequences, raising the question of whether they contain xrRNAs and if so, how they fold. To answer this, we demonstrated the presence of an authentic xrRNA in the 3' UTR of the Tamana Bat Virus (TABV) and solved its structure by x-ray crystallography. The structure reveals conserved features from previously characterized xrRNAs, but in the TABV version these features are created through a novel set of tertiary interactions not previously seen in xrRNAs. This includes two important A-C interactions, four distinct backbone kinks, several ordered Mg2+ ions, and a C+-G-C base triple. The discovery that the same overall architecture can be achieved by very different sequences and interactions in distantly related flaviviruses provides insight into the diversity of this type of RNA and will inform searches for undiscovered xrRNAs in viruses and beyond.
Different tertiary interactions create the same important 3-D features in a distinct flavivirus xrRNA.,Jones RA, Steckelberg AL, Vicens Q, Szucs MJ, Akiyama BM, Kieft JS RNA. 2020 Oct 1. pii: rna.077065.120. doi: 10.1261/rna.077065.120. PMID:33004436[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jones RA, Steckelberg AL, Vicens Q, Szucs MJ, Akiyama BM, Kieft JS. Different tertiary interactions create the same important 3-D features in a distinct flavivirus xrRNA. RNA. 2020 Oct 1. pii: rna.077065.120. doi: 10.1261/rna.077065.120. PMID:33004436 doi:http://dx.doi.org/10.1261/rna.077065.120
|
