7kgm
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==C. rodentium YcbB - ertapenem complex== | ==C. rodentium YcbB - ertapenem complex== | ||
| - | <StructureSection load='7kgm' size='340' side='right'caption='[[7kgm]]' scene=''> | + | <StructureSection load='7kgm' size='340' side='right'caption='[[7kgm]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KGM OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7kgm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_rodentium_ICC168 Citrobacter rodentium ICC168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KGM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1RG:(4R,5S)-3-({(3S,5S)-5-[(3-CARBOXYPHENYL)CARBAMOYL]PYRROLIDIN-3-YL}SULFANYL)-5-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-4-METHYL-4,5-DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>1RG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kgm OCA], [https://pdbe.org/7kgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kgm RCSB], [https://www.ebi.ac.uk/pdbsum/7kgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kgm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D2TS62_CITRI D2TS62_CITRI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The bacterial cell wall plays a key role in viability and is an important drug target. The cell wall is made of elongated polymers that are crosslinked to one another to form a load bearing mesh. An alternate cell wall crosslinking mechanism performed by the L,D-transpeptidase YcbB has been implicated in the stress regulated roles of beta-lactam resistance, outer membrane defect rescue, and typhoid toxin release. The role for this stress linked crosslinking in the context of a host infection was unclear. Here we resolve the crystallographic structures of both S Typhi YcbB and C. rodentium YcbB acylated with ertapenem that delineate the conserved structural characteristics of YcbB. In parallel, we show that the general role of YcbB in peptidoglycan reinforcement under bacterial outer envelope stress does not play a significant role in acute infections of mice by C. rodentium and S Typhimurium. Cumulatively, in this work we provide a foundation for the development of novel YcbB specific antibacterial therapeutics to assist in treatment of increasingly drug resistant S Typhi infections. | ||
| + | |||
| + | Structural and cellular insights into the L,D-transpeptidase YcbB as a therapeutic target in C. rodentium, S. Typhimurium, and S. Typhi infections.,Caveney NA, Serapio-Palacios A, Woodward SE, Bozorgmehr T, Caballero G, Vuckovic M, Deng W, Finlay BB, Strynadka NCJ Antimicrob Agents Chemother. 2020 Nov 2. pii: AAC.01592-20. doi:, 10.1128/AAC.01592-20. PMID:33139287<ref>PMID:33139287</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7kgm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Citrobacter rodentium ICC168]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Caveney NA]] | [[Category: Caveney NA]] | ||
[[Category: Strynadka NCJ]] | [[Category: Strynadka NCJ]] | ||
Revision as of 15:24, 18 October 2023
C. rodentium YcbB - ertapenem complex
| |||||||||||
