7kik

==Wheat dwarf virus Rep domain circular permutation complexed with a single-stranded DNA 10-mer comprising the cleavage site and Mn2+==

<StructureSection load='7kik' size='340' side='right'caption='[[7kik]], [[Resolution|resolution]] 1.73&Aring;' scene=''>

<table><tr><td colspan='2'>[[7kik]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Wheat_dwarf_virus Wheat dwarf virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIK FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kik OCA], [https://pdbe.org/7kik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kik RCSB], [https://www.ebi.ac.uk/pdbsum/7kik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kik ProSAT]</span></td></tr>

== Function ==

[https://www.uniprot.org/uniprot/REP_WDVS REP_WDVS] Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. Acts as an inhibitor of C-sense gene transcription (By similarity).

[[Category: Wheat dwarf virus]]

[[Category: Tompkins KJ]]