7l7r
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs== |
| - | <StructureSection load='7l7r' size='340' side='right'caption='[[7l7r]]' scene=''> | + | <StructureSection load='7l7r' size='340' side='right'caption='[[7l7r]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7l7r]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Crimean-Congo_hemorrhagic_fever_virus_strain_IbAr10200 Crimean-Congo hemorrhagic fever virus strain IbAr10200] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L7R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l7r OCA], [https://pdbe.org/7l7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l7r RCSB], [https://www.ebi.ac.uk/pdbsum/7l7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l7r ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l7r OCA], [https://pdbe.org/7l7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l7r RCSB], [https://www.ebi.ac.uk/pdbsum/7l7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l7r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/GP_CCHFI GP_CCHFI] Glycoprotein C and glycoprotein N interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion (By similarity).<ref>PMID:19088291</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Crimean-Congo hemorrhagic fever virus (CCHFV) is the most widespread tick-borne zoonotic virus, with a 30% case fatality rate in humans. Structural information is lacking in regard to the CCHFV membrane fusion glycoprotein Gc-the main target of the host neutralizing antibody response-as well as antibody-mediated neutralization mechanisms. We describe the structure of prefusion Gc bound to the antigen-binding fragments (Fabs) of two neutralizing antibodies that display synergy when combined, as well as the structure of trimeric, postfusion Gc. The structures show the two Fabs acting in concert to block membrane fusion, with one targeting the fusion loops and the other blocking Gc trimer formation. The structures also revealed the neutralization mechanism of previously reported antibodies against CCHFV, providing the molecular underpinnings essential for developing CCHFV-specific medical countermeasures for epidemic preparedness. | ||
| + | |||
| + | Structural basis of synergistic neutralization of Crimean-Congo hemorrhagic fever virus by human antibodies.,Mishra AK, Hellert J, Freitas N, Guardado-Calvo P, Haouz A, Fels JM, Maurer DP, Abelson DM, Bornholdt ZA, Walker LM, Chandran K, Cosset FL, McLellan JS, Rey FA Science. 2022 Jan 7;375(6576):104-109. doi: 10.1126/science.abl6502. Epub 2021, Nov 18. PMID:34793197<ref>PMID:34793197</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7l7r" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Crimean-Congo hemorrhagic fever virus strain IbAr10200]] | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: McLellan JS]] |
| + | [[Category: Mishra AK]] | ||
Current revision
CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs
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