7md0
From Proteopedia
(Difference between revisions)
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==Crystal structure of Staphylococcus aureus cystathionine gamma lyase holoenzyme in the presence of NL1F3== | ==Crystal structure of Staphylococcus aureus cystathionine gamma lyase holoenzyme in the presence of NL1F3== | ||
| - | <StructureSection load='7md0' size='340' side='right'caption='[[7md0]]' scene=''> | + | <StructureSection load='7md0' size='340' side='right'caption='[[7md0]], [[Resolution|resolution]] 2.12Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MD0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7md0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MD0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7md0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7md0 OCA], [https://pdbe.org/7md0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7md0 RCSB], [https://www.ebi.ac.uk/pdbsum/7md0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7md0 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7md0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7md0 OCA], [https://pdbe.org/7md0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7md0 RCSB], [https://www.ebi.ac.uk/pdbsum/7md0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7md0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0H3K724_STAAE A0A0H3K724_STAAE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Emergent resistance to all clinical antibiotics calls for the next generation of therapeutics. Here we report an effective antimicrobial strategy targeting the bacterial hydrogen sulfide (H2S)-mediated defense system. We identified cystathionine gamma-lyase (CSE) as the primary generator of H2S in two major human pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, and discovered small molecules that inhibit bacterial CSE. These inhibitors potentiate bactericidal antibiotics against both pathogens in vitro and in mouse models of infection. CSE inhibitors also suppress bacterial tolerance, disrupting biofilm formation and substantially reducing the number of persister bacteria that survive antibiotic treatment. Our results establish bacterial H2S as a multifunctional defense factor and CSE as a drug target for versatile antibiotic enhancers. | ||
| + | |||
| + | Inhibitors of bacterial H2S biogenesis targeting antibiotic resistance and tolerance.,Shatalin K, Nuthanakanti A, Kaushik A, Shishov D, Peselis A, Shamovsky I, Pani B, Lechpammer M, Vasilyev N, Shatalina E, Rebatchouk D, Mironov A, Fedichev P, Serganov A, Nudler E Science. 2021 Jun 11;372(6547):1169-1175. doi: 10.1126/science.abd8377. PMID:34112687<ref>PMID:34112687</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7md0" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cystathionine beta-lyase|Cystathionine beta-lyase]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus]] | ||
[[Category: Kaushik A]] | [[Category: Kaushik A]] | ||
[[Category: Nuthanakanti A]] | [[Category: Nuthanakanti A]] | ||
[[Category: Serganov A]] | [[Category: Serganov A]] | ||
Current revision
Crystal structure of Staphylococcus aureus cystathionine gamma lyase holoenzyme in the presence of NL1F3
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