7mk0
From Proteopedia
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==Trypanosoma cruzi Nucleoside Diphosphate Kinase 1 form a quinary multihexameric structure== | ==Trypanosoma cruzi Nucleoside Diphosphate Kinase 1 form a quinary multihexameric structure== | ||
| - | <StructureSection load='7mk0' size='340' side='right'caption='[[7mk0]]' scene=''> | + | <StructureSection load='7mk0' size='340' side='right'caption='[[7mk0]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MK0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7mk0]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MK0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mk0 OCA], [https://pdbe.org/7mk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mk0 RCSB], [https://www.ebi.ac.uk/pdbsum/7mk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mk0 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mk0 OCA], [https://pdbe.org/7mk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mk0 RCSB], [https://www.ebi.ac.uk/pdbsum/7mk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mk0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q4E256_TRYCC Q4E256_TRYCC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Trypanosoma cruzi is a flagellated protozoan parasite that causes Chagas disease, which represents a serious health problem in the Americas. Nucleoside diphosphate kinases (NDPKs) are key enzymes that are implicated in cellular energy management. TcNDPK1 is the canonical isoform in the T. cruzi parasite. TcNDPK1 has a cytosolic, perinuclear and nuclear distribution. It is also found in non-membrane-bound filaments adjacent to the nucleus. In the present work, X-ray diffraction and in vivo studies of TcNDPK1 are described. The structure reveals a novel, multi-hexameric, left-handed helical oligomer structure. The results of directed mutagenesis studies led to the conclusion that the microscopic TcNDPK1 granules observed in vivo in T. cruzi parasites are made up by the association of TcNDPK1 oligomers. In the absence of experimental data, analysis of the interactions in the X-ray structure of the TcNDPK1 oligomer suggests the probable assembly and disassembly steps: dimerization, assembly of the hexamer as a trimer of dimers, hexamer association to generate the left-handed helical oligomer structure and finally oligomer association in a parallel manner to form the microscopic TcNDPK1 filaments that are observed in vivo in T. cruzi parasites. Oligomer disassembly takes place on the binding of substrate in the active site of TcNDPK1, leading to dissociation of the hexamers. This study constitutes the first report of such a protein arrangement, which has never previously been seen for any protein or NDPK. Further studies are needed to determine its physiological role. However, it may suggest a paradigm for protein storage reflecting the complex mechanism of action of TcNDPK1. | ||
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| + | X-ray diffraction and in vivo studies reveal the quinary structure of Trypanosoma cruzi nucleoside diphosphate kinase 1: a novel helical oligomer structure.,Gomez Barroso JA, Miranda MR, Pereira CA, Garratt RC, Aguilar CF Acta Crystallogr D Struct Biol. 2022 Jan 1;78(Pt 1):30-42. doi:, 10.1107/S2059798321011219. Epub 2022 Jan 1. PMID:34981759<ref>PMID:34981759</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7mk0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Trypanosoma cruzi]] | ||
[[Category: Aguilar CF]] | [[Category: Aguilar CF]] | ||
[[Category: Gomez JA]] | [[Category: Gomez JA]] | ||
Current revision
Trypanosoma cruzi Nucleoside Diphosphate Kinase 1 form a quinary multihexameric structure
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