1nlu

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[[Image:1nlu.jpg|left|200px]]
[[Image:1nlu.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1nlu |SIZE=350|CAPTION= <scene name='initialview01'>1nlu</scene>, resolution 1.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1nlu", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IVA:ISOVALERIC+ACID'>IVA</scene>, <scene name='pdbligand=PHI:IODO-PHENYLALANINE'>PHI</scene>, <scene name='pdbligand=TYB:TYROSINAL'>TYB</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pseudomonalisin Pseudomonalisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.100 3.4.21.100] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= PCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.])
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|DOMAIN=
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{{STRUCTURE_1nlu| PDB=1nlu | SCENE= }}
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|RELATEDENTRY=[[1ga6|1GA6]], [[1kdv|1KDV]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nlu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nlu OCA], [http://www.ebi.ac.uk/pdbsum/1nlu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nlu RCSB]</span>
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}}
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'''Pseudomonas sedolisin (serine-carboxyl proteinase) complexed with two molecules of pseudo-iodotyrostatin'''
'''Pseudomonas sedolisin (serine-carboxyl proteinase) complexed with two molecules of pseudo-iodotyrostatin'''
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[[Category: Uchida, K.]]
[[Category: Uchida, K.]]
[[Category: Wlodawer, A.]]
[[Category: Wlodawer, A.]]
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[[Category: pscp]]
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[[Category: Pscp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:41:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:32:07 2008''
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Revision as of 23:41, 2 May 2008

Image:1nlu.jpg

Template:STRUCTURE 1nlu

Pseudomonas sedolisin (serine-carboxyl proteinase) complexed with two molecules of pseudo-iodotyrostatin


Overview

High-resolution crystallographic analysis of a complex of the serine-carboxyl proteinase sedolisin with pseudo-iodotyrostatin revealed two molecules of this inhibitor bound in the active site of the enzyme, marking subsites from S3 to S3('). The mode of binding represents two products of the proteolytic reaction. Substrate specificity of sedolisin was investigated using peptide libraries and a new peptide substrate for sedolisin, MCA-Lys-Pro-Pro-Leu-Glu#Tyr-Arg-Leu-Gly-Lys(DNP)-Gly, was synthesized based on the results of the enzymatic and crystallographic studies and was shown to be efficiently cleaved by the enzyme. The kinetic parameters for the substrate, measured by the increase in fluorescence upon relief of quenching, were: k(cat)=73+/-5 s(-1), K(m)=0.12+/-0.011 microM, and k(cat)/K(m)=608+/-85 s(-1)microM(-1).

About this Structure

1NLU is a Single protein structure of sequence from Pseudomonas sp.. Full crystallographic information is available from OCA.

Reference

Two inhibitor molecules bound in the active site of Pseudomonas sedolisin: a model for the bi-product complex following cleavage of a peptide substrate., Wlodawer A, Li M, Gustchina A, Oyama H, Oda K, Beyer BB, Clemente J, Dunn BM, Biochem Biophys Res Commun. 2004 Feb 6;314(2):638-45. PMID:14733955 Page seeded by OCA on Sat May 3 02:41:03 2008

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