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7r8w
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Crystal Structure of the LNK SH2 Domain in Complex with a JAK2 pY813 Phosphopeptide== |
| - | <StructureSection load='7r8w' size='340' side='right'caption='[[7r8w]]' scene=''> | + | <StructureSection load='7r8w' size='340' side='right'caption='[[7r8w]], [[Resolution|resolution]] 1.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7r8w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R8W FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r8w OCA], [https://pdbe.org/7r8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r8w RCSB], [https://www.ebi.ac.uk/pdbsum/7r8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r8w ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r8w OCA], [https://pdbe.org/7r8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r8w RCSB], [https://www.ebi.ac.uk/pdbsum/7r8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r8w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SH2B3_MOUSE SH2B3_MOUSE] Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The SH2B family of adaptor proteins, SH2-B, APS, and LNK are key modulators of cellular signalling pathways. Whilst SH2-B and APS have been partially structurally and biochemically characterised, to date there has been no such characterisation of LNK. Here we present two crystal structures of the LNK substrate recognition domain, the SH2 domain, bound to phosphorylated motifs from JAK2 and EPOR, and biochemically define the basis for target recognition. The LNK SH2 domain adopts a canonical SH2 domain fold with an additional N-terminal helix. Targeted analysis of binding to phosphosites in signalling pathways indicated that specificity is conferred by amino acids one- and three-residues downstream of the phosphotyrosine. Several mutations in LNK showed impaired target binding in vitro and a reduced ability to inhibit signalling, allowing an understanding of the molecular basis of LNK dysfunction in variants identified in patients with myeloproliferative disease. | ||
| + | |||
| + | Structural and functional analysis of target recognition by the lymphocyte adaptor protein LNK.,Morris R, Zhang Y, Ellyard JI, Vinuesa CG, Murphy JM, Laktyushin A, Kershaw NJ, Babon JJ Nat Commun. 2021 Oct 20;12(1):6110. doi: 10.1038/s41467-021-26394-6. PMID:34671038<ref>PMID:34671038</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7r8w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| + | [[Category: Babon JJ]] | ||
| + | [[Category: Kershaw NJ]] | ||
| + | [[Category: Morris R]] | ||
Current revision
Crystal Structure of the LNK SH2 Domain in Complex with a JAK2 pY813 Phosphopeptide
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