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| | == Structural highlights == | | == Structural highlights == |
| | <table><tr><td colspan='2'>[[7r97]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R97 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[7r97]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R97 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.804Å</td></tr> |
| | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r97 OCA], [https://pdbe.org/7r97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r97 RCSB], [https://www.ebi.ac.uk/pdbsum/7r97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r97 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r97 OCA], [https://pdbe.org/7r97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r97 RCSB], [https://www.ebi.ac.uk/pdbsum/7r97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r97 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/RNC_ECOLI RNC_ECOLI]] Digests double-stranded RNA formed within single-strand substrates, but not RNA-DNA hybrids. Involved in the processing of rRNA precursors, viral transcripts, some mRNAs and at least 1 tRNA (metY, a minor form of tRNA-init-Met). Cleaves the 30S primary rRNA transcript to yield the immediate precursors to the 16S and 23S rRNAs; cleavage can occur in assembled 30S, 50S and even 70S subunits and is influenced by the presence of ribosomal proteins. The E.coli enzyme does not cleave R.capsulatus rRNA precursor, although R.capsulatus will complement an E.coli disruption, showing substrate recognition is different. Removes the intervening sequences from Salmonella typhimurium rRNA precursor. Complements the pre-crRNA processing defect in an rnc deletion in S.pyogenes strain 370, although this E.coli strain does not have the corresponding CRISPR locus (strain TOP10) (PubMed:23535272).<ref>PMID:2085545</ref> <ref>PMID:23535272</ref> <ref>PMID:2406020</ref> <ref>PMID:2481042</ref> <ref>PMID:3903434</ref> <ref>PMID:4587248</ref> <ref>PMID:4592261</ref> <ref>PMID:4610145</ref> <ref>PMID:4865702</ref> <ref>PMID:6159890</ref> <ref>PMID:932008</ref>
| + | [https://www.uniprot.org/uniprot/RNC_ECOLI RNC_ECOLI] Digests double-stranded RNA formed within single-strand substrates, but not RNA-DNA hybrids. Involved in the processing of rRNA precursors, viral transcripts, some mRNAs and at least 1 tRNA (metY, a minor form of tRNA-init-Met). Cleaves the 30S primary rRNA transcript to yield the immediate precursors to the 16S and 23S rRNAs; cleavage can occur in assembled 30S, 50S and even 70S subunits and is influenced by the presence of ribosomal proteins. The E.coli enzyme does not cleave R.capsulatus rRNA precursor, although R.capsulatus will complement an E.coli disruption, showing substrate recognition is different. Removes the intervening sequences from Salmonella typhimurium rRNA precursor. Complements the pre-crRNA processing defect in an rnc deletion in S.pyogenes strain 370, although this E.coli strain does not have the corresponding CRISPR locus (strain TOP10) (PubMed:23535272).<ref>PMID:2085545</ref> <ref>PMID:23535272</ref> <ref>PMID:2406020</ref> <ref>PMID:2481042</ref> <ref>PMID:3903434</ref> <ref>PMID:4587248</ref> <ref>PMID:4592261</ref> <ref>PMID:4610145</ref> <ref>PMID:4865702</ref> <ref>PMID:6159890</ref> <ref>PMID:932008</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Structural highlights
7r97 is a 4 chain structure with sequence from Escherichia coli and Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.804Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
RNC_ECOLI Digests double-stranded RNA formed within single-strand substrates, but not RNA-DNA hybrids. Involved in the processing of rRNA precursors, viral transcripts, some mRNAs and at least 1 tRNA (metY, a minor form of tRNA-init-Met). Cleaves the 30S primary rRNA transcript to yield the immediate precursors to the 16S and 23S rRNAs; cleavage can occur in assembled 30S, 50S and even 70S subunits and is influenced by the presence of ribosomal proteins. The E.coli enzyme does not cleave R.capsulatus rRNA precursor, although R.capsulatus will complement an E.coli disruption, showing substrate recognition is different. Removes the intervening sequences from Salmonella typhimurium rRNA precursor. Complements the pre-crRNA processing defect in an rnc deletion in S.pyogenes strain 370, although this E.coli strain does not have the corresponding CRISPR locus (strain TOP10) (PubMed:23535272).[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
References
- ↑ Regnier P, Grunberg-Manago M. RNase III cleavages in non-coding leaders of Escherichia coli transcripts control mRNA stability and genetic expression. Biochimie. 1990 Nov;72(11):825-34. doi: 10.1016/0300-9084(90)90192-j. PMID:2085545 doi:http://dx.doi.org/10.1016/0300-9084(90)90192-j
- ↑ Karvelis T, Gasiunas G, Miksys A, Barrangou R, Horvath P, Siksnys V. crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus. RNA Biol. 2013 May;10(5):841-51. doi: 10.4161/rna.24203. Epub 2013 Mar 27. PMID:23535272 doi:http://dx.doi.org/10.4161/rna.24203
- ↑ Burgin AB, Parodos K, Lane DJ, Pace NR. The excision of intervening sequences from Salmonella 23S ribosomal RNA. Cell. 1990 Feb 9;60(3):405-14. doi: 10.1016/0092-8674(90)90592-3. PMID:2406020 doi:http://dx.doi.org/10.1016/0092-8674(90)90592-3
- ↑ Regnier P, Grunberg-Manago M. Cleavage by RNase III in the transcripts of the met Y-nus-A-infB operon of Escherichia coli releases the tRNA and initiates the decay of the downstream mRNA. J Mol Biol. 1989 Nov 20;210(2):293-302. doi: 10.1016/0022-2836(89)90331-8. PMID:2481042 doi:http://dx.doi.org/10.1016/0022-2836(89)90331-8
- ↑ Nashimoto H, Uchida H. DNA sequencing of the Escherichia coli ribonuclease III gene and its mutations. Mol Gen Genet. 1985;201(1):25-9. doi: 10.1007/BF00397981. PMID:3903434 doi:http://dx.doi.org/10.1007/BF00397981
- ↑ Dunn JJ, Studier FW. T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3296-3300. doi:, 10.1073/pnas.70.12.3296. PMID:4587248 doi:http://dx.doi.org/10.1073/pnas.70.12.3296
- ↑ Crouch RJ. Ribonuclease 3 does not degrade deoxyribonucleic acid-ribonucleic acid hybrids. J Biol Chem. 1974 Feb 25;249(4):1314-6. PMID:4592261
- ↑ Nikolaev N, Schlessinger D, Wellauer PK. 30 S pre-ribosomal RNA of Escherichia coli and products of cleavage by ribonuclease III: length and molecular weight. J Mol Biol. 1974 Jul 15;86(4):741-7. doi: 10.1016/0022-2836(74)90350-7. PMID:4610145 doi:http://dx.doi.org/10.1016/0022-2836(74)90350-7
- ↑ Robertson HD, Webster RE, Zinder ND. Purification and properties of ribonuclease III from Escherichia coli. J Biol Chem. 1968 Jan 10;243(1):82-91. PMID:4865702
- ↑ Gitelman DR, Apirion D. The synthesis of some proteins is affected in RNA processing mutants of Escherichia coli. Biochem Biophys Res Commun. 1980 Oct 16;96(3):1063-70. doi:, 10.1016/0006-291x(80)90060-1. PMID:6159890 doi:http://dx.doi.org/10.1016/0006-291x(80)90060-1
- ↑ Dunn JJ. RNase III cleavage of single-stranded RNA. Effect of ionic strength on the fideltiy of cleavage. J Biol Chem. 1976 Jun 25;251(12):3807-14. PMID:932008
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