7rlm
From Proteopedia
(Difference between revisions)
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==Crystal Structure of Potato Leafroll Virus (PLRV) Coat Protein== | ==Crystal Structure of Potato Leafroll Virus (PLRV) Coat Protein== | ||
| - | <StructureSection load='7rlm' size='340' side='right'caption='[[7rlm]]' scene=''> | + | <StructureSection load='7rlm' size='340' side='right'caption='[[7rlm]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RLM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RLM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7rlm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Potato_leafroll_virus Potato leafroll virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RLM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RLM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rlm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rlm OCA], [https://pdbe.org/7rlm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rlm RCSB], [https://www.ebi.ac.uk/pdbsum/7rlm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rlm ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rlm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rlm OCA], [https://pdbe.org/7rlm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rlm RCSB], [https://www.ebi.ac.uk/pdbsum/7rlm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rlm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CAPSD_PLRV1 CAPSD_PLRV1] Major capsid protein that self-assembles to form an icosahedral capsid with a T=3 symmetry, about 23 nm in diameter, and consisting of 180 capsid proteins monomers (PubMed:31611039, PubMed:34813955). Most of the 180 monomers are the major capsid protein, but a small percentage contain the minor capsid protein, which has a long C-terminal extension (PubMed:31611039).<ref>PMID:31611039</ref> <ref>PMID:34813955</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Luteoviruses, poleroviruses, and enamoviruses are insect-transmitted, agricultural pathogens that infect a wide array of plants, including staple food crops. Previous cryo-electron microscopy studies of virus-like particles show that luteovirid viral capsids are built from a structural coat protein that organizes with T = 3 icosahedral symmetry. Here, we present the crystal structure of a truncated version of the coat protein monomer from potato leafroll virus at 1.80-A resolution. In the crystal lattice, monomers pack into flat sheets that preserve the two-fold and three-fold axes of icosahedral symmetry and show minimal structural deviations when compared to the full-length subunits of the assembled virus-like particle. These observations have important implications in viral assembly and maturation and suggest that the CP N-terminus and its interactions with RNA play an important role in generating capsid curvature. | ||
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| + | Crystal structure of the potato leafroll virus coat protein and implications for viral assembly.,Adams MC, Schiltz CJ, Heck ML, Chappie JS J Struct Biol. 2021 Nov 20;214(1):107811. doi: 10.1016/j.jsb.2021.107811. PMID:34813955<ref>PMID:34813955</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7rlm" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Potato leafroll virus]] | ||
[[Category: Adams MC]] | [[Category: Adams MC]] | ||
[[Category: Chappie JS]] | [[Category: Chappie JS]] | ||
[[Category: Schiltz CJ]] | [[Category: Schiltz CJ]] | ||
Current revision
Crystal Structure of Potato Leafroll Virus (PLRV) Coat Protein
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