1nm7
From Proteopedia
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[[Image:1nm7.gif|left|200px]] | [[Image:1nm7.gif|left|200px]] | ||
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'''Solution structure of the ScPex13p SH3 domain''' | '''Solution structure of the ScPex13p SH3 domain''' | ||
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[[Category: Schneider-Mergener, J.]] | [[Category: Schneider-Mergener, J.]] | ||
[[Category: Volkmer-Engert, R.]] | [[Category: Volkmer-Engert, R.]] | ||
| - | [[Category: | + | [[Category: Import machine]] |
| - | [[Category: | + | [[Category: Membrane protein]] |
| - | [[Category: | + | [[Category: Pex13p]] |
| - | [[Category: | + | [[Category: Pex14p]] |
| - | [[Category: | + | [[Category: Pex5p]] |
| - | [[Category: | + | [[Category: Sh3 domain]] |
| - | [[Category: | + | [[Category: Yeast]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:41:56 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:41, 2 May 2008
Solution structure of the ScPex13p SH3 domain
Overview
Pex13p is an essential component of the peroxisomal protein import machinery and interacts via its C-terminal SH3 domain with the type II SH3-ligand Pex14p and the non-PXXP protein Pex5p. We report the solution structure of the SH3 domain of Pex13p from Saccharomyces cerevisiae and the identification of a novel-binding pocket, which binds a non-PXXP-peptide representing the binding site of Pex5p. Chemical shift assays revealed the binding sites for Pex5p and Pex14p ligand peptides to be distinct and spatially separated. Competition assays demonstrated that the two ligand peptides can bind simultaneously to the SH3 domain.
About this Structure
1NM7 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The ScPex13p SH3 domain exposes two distinct binding sites for Pex5p and Pex14p., Pires JR, Hong X, Brockmann C, Volkmer-Engert R, Schneider-Mergener J, Oschkinat H, Erdmann R, J Mol Biol. 2003 Mar 7;326(5):1427-35. PMID:12595255 Page seeded by OCA on Sat May 3 02:41:56 2008
