7s4w

From Proteopedia

(Difference between revisions)

Revision as of 16:44, 18 October 2023

Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Lysozyme

Structural highlights

7s4w is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Publication Abstract from PubMed

The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15-30 microm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and alpha-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 microm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures.

Serial macromolecular crystallography at ALBA Synchrotron Light Source.,Martin-Garcia JM, Botha S, Hu H, Jernigan R, Castellvi A, Lisova S, Gil F, Calisto B, Crespo I, Roy-Chowdhury S, Grieco A, Ketawala G, Weierstall U, Spence J, Fromme P, Zatsepin N, Boer DR, Carpena X J Synchrotron Radiat. 2022 May 1;29(Pt 3):896-907. doi:, 10.1107/S1600577522002508. Epub 2022 Apr 4. PMID:35511023^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Martin-Garcia JM, Botha S, Hu H, Jernigan R, Castellvi A, Lisova S, Gil F, Calisto B, Crespo I, Roy-Chowdhury S, Grieco A, Ketawala G, Weierstall U, Spence J, Fromme P, Zatsepin N, Boer DR, Carpena X. Serial macromolecular crystallography at ALBA Synchrotron Light Source. J Synchrotron Radiat. 2022 May 1;29(Pt 3):896-907. doi:, 10.1107/S1600577522002508. Epub 2022 Apr 4. PMID:35511023 doi:http://dx.doi.org/10.1107/S1600577522002508

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7s4w"

@@ Line 3: / Line 3: @@
 <StructureSection load='7s4w' size='340' side='right'caption='[[7s4w]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7s4w]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S4W FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7s4w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S4W FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7s4r|7s4r]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s4w OCA], [https://pdbe.org/7s4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s4w RCSB], [https://www.ebi.ac.uk/pdbsum/7s4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s4w ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
+[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 19: @@
 </div>
 <div class="pdbe-citations 7s4w" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Gallus gallus]]
 [[Category: Large Structures]]
-[[Category: Lysozyme]]
+[[Category: Boer R]]
-[[Category: Boer, R]]
+[[Category: Botha S]]
-[[Category: Botha, S]]
+[[Category: Calisto B]]
-[[Category: Calisto, B]]
+[[Category: Carpena X]]
-[[Category: Carpena, X]]
+[[Category: Castellvi A]]
-[[Category: Castellvi, A]]
+[[Category: Crespo I]]
-[[Category: Crespo, I]]
+[[Category: Fromme P]]
-[[Category: Fromme, P]]
+[[Category: Gil F]]
-[[Category: Gil, F]]
+[[Category: Grieco A]]
-[[Category: Grieco, A]]
+[[Category: Hu H]]
-[[Category: Hu, H]]
+[[Category: Jernigan R]]
-[[Category: Jernigan, R]]
+[[Category: Ketawala G]]
-[[Category: Ketawala, G]]
+[[Category: Lisova S]]
-[[Category: Lisova, S]]
+[[Category: Martin-Garcia JM]]
-[[Category: Martin-Garcia, J M]]
+[[Category: Roy-Chowdbury S]]
-[[Category: Roy-Chowdbury, S]]
+[[Category: Spence J]]
-[[Category: Spence, J]]
+[[Category: Weierstall U]]
-[[Category: Weierstall, U]]
+[[Category: Zatsepin N]]
-[[Category: Zatsepin, N]]
-[[Category: Hydrolase]]
-[[Category: Serial crystallography]]

7s4w

From Proteopedia

Revision as of 16:44, 18 October 2023

Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Lysozyme

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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