7s7q

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==Heterodimeric complex of Pf12 and Pf41 of Plasmodium falciparum==
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<StructureSection load='7s7q' size='340' side='right'caption='[[7s7q]]' scene=''>
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<StructureSection load='7s7q' size='340' side='right'caption='[[7s7q]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7s7q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S7Q FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7q OCA], [https://pdbe.org/7s7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7q RCSB], [https://www.ebi.ac.uk/pdbsum/7s7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7q ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s7q OCA], [https://pdbe.org/7s7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s7q RCSB], [https://www.ebi.ac.uk/pdbsum/7s7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s7q ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PF41_PLAF7 PF41_PLAF7]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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During the different stages of the Plasmodium life cycle, surface-associated proteins establish key interactions with the host and play critical roles in parasite survival. The 6-cysteine (6-cys) protein family is one of the most abundant surface antigens and expressed throughout the Plasmodium falciparum life cycle. This protein family is conserved across Plasmodium species and plays critical roles in parasite transmission, evasion of the host immune response and host cell invasion. Several 6-cys proteins are present on the parasite surface as hetero-complexes but it is not known how two 6-cys proteins interact together. Here, we present a crystal structure of Pf12 bound to Pf41 at 2.85 A resolution, two P. falciparum proteins usually found on the parasite surface of late schizonts and merozoites. Our structure revealed two critical interfaces required for complex formation with important implications on how different 6-cysteine proteins may interact with each other. Using structure-function analyses, we identified important residues for Pf12-Pf41 complex formation. In addition, we generated 16 nanobodies against Pf12 and Pf41 and showed that several Pf12-specific nanobodies inhibit Pf12-Pf41 complex formation. Using X-ray crystallography, we were able to describe the structural mechanism of an inhibitory nanobody in blocking Pf12-Pf41 complex formation. Future studies using these inhibitory nanobodies will be useful to determine the functional role of these two 6-cys proteins in malaria parasites.
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Structure of the Pf12 and Pf41 heterodimeric complex of Plasmodium falciparum 6-cysteine proteins.,Dietrich MH, Chan LJ, Adair A, Boulet C, O'Neill MT, Tan LL, Keremane S, Mok YF, Lo AW, Gilson P, Tham WH FEMS Microbes. 2022 Feb 16;3:xtac005. doi: 10.1093/femsmc/xtac005. eCollection , 2022. PMID:35308105<ref>PMID:35308105</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7s7q" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Z-disk]]
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Dietrich MH]]
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[[Category: Tham WH]]

Revision as of 16:46, 18 October 2023

Heterodimeric complex of Pf12 and Pf41 of Plasmodium falciparum

PDB ID 7s7q

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