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7sbg
From Proteopedia
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| - | ==== | + | ==Murine Fab/IgE in complex with profilin from Hevea brasieliensis (Hev b 8)== |
| - | <StructureSection load='7sbg' size='340' side='right'caption='[[7sbg]]' scene=''> | + | <StructureSection load='7sbg' size='340' side='right'caption='[[7sbg]], [[Resolution|resolution]] 3.34Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7sbg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SBG FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sbg OCA], [https://pdbe.org/7sbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sbg RCSB], [https://www.ebi.ac.uk/pdbsum/7sbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sbg ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.34Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sbg OCA], [https://pdbe.org/7sbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sbg RCSB], [https://www.ebi.ac.uk/pdbsum/7sbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sbg ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PROF2_HEVBR PROF2_HEVBR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Allergies have become a rising health problem, where plentiful substances can trigger IgE-mediated allergies in humans. While profilins are considered minor allergens, these ubiquitous proteins are primary molecules involved in cross-reactivity and pollen-food allergy syndrome. Here we report the first crystal structures of murine Fab/IgE, with its chains naturally paired, in complex with the allergen profilin from Hevea brasiliensis (Hev b 8). The crystallographic models revealed that the IgE's six complementarity-determining regions (CDRs) interact with the allergen, comprising a rigid paratope-epitope surface of 926 A(2), which includes an extensive network of interactions. Interestingly, we also observed previously unreported flexibility at Fab/IgE's elbow angle, which did not influence the shape of the paratope. The Fab/IgE exhibits a high affinity for Hev b 8, even when using 1 M NaCl in BLI experiments. Finally, based on the encouraging cross-reactivity assays using two mutants of the maize profilin (Zea m 12), this antibody could be a promising tool in IgE engineering for diagnosis and research applications. | ||
| + | |||
| + | A native IgE in complex with profilin provides insights into allergen recognition and cross-reactivity.,Garcia-Ramirez B, Mares-Mejia I, Rodriguez-Hernandez A, Cano-Sanchez P, Torres-Larios A, Ortega E, Rodriguez-Romero A Commun Biol. 2022 Jul 27;5(1):748. doi: 10.1038/s42003-022-03718-w. PMID:35902770<ref>PMID:35902770</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7sbg" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | *[[Profilin 3D Structures|Profilin 3D Structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Hevea brasiliensis]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| + | [[Category: Garcia-Ramirez B]] | ||
| + | [[Category: Rodriguez-Romero A]] | ||
Current revision
Murine Fab/IgE in complex with profilin from Hevea brasieliensis (Hev b 8)
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