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2agn

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Revision as of 18:07, 18 October 2023

Fitting of hepatitis C virus internal ribosome entry site domains into the 15 A Cryo-EM map of a HCV IRES-80S ribosome (H. sapiens) complex

2agn, resolution 15.00Å

Structural highlights

2agn is a 5 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 15Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Initiation of translation of the hepatitis C virus (HCV) polyprotein is driven by an internal ribosome entry site (IRES) RNA that bypasses much of the eukaryotic translation initiation machinery. Here, single-particle electron cryomicroscopy has been used to study the mechanism of HCV IRES-mediated initiation. A HeLa in vitro translation system was used to assemble human IRES-80S ribosome complexes under near physiological conditions; these were stalled before elongation. Domain 2 of the HCV IRES is bound to the tRNA exit site, touching the L1 stalk of the 60S subunit, suggesting a mechanism for the removal of the HCV IRES in the progression to elongation. Domain 3 of the HCV IRES positions the initiation codon in the ribosomal mRNA binding cleft by binding helix 28 at the head of the 40S subunit. The comparison with the previously published binary 40S-HCV IRES complex reveals structural rearrangements in the two pseudoknot structures of the HCV IRES in translation initiation.

Structure of the hepatitis C virus IRES bound to the human 80S ribosome: remodeling of the HCV IRES.,Boehringer D, Thermann R, Ostareck-Lederer A, Lewis JD, Stark H Structure. 2005 Nov;13(11):1695-706. PMID:16271893^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Boehringer D, Thermann R, Ostareck-Lederer A, Lewis JD, Stark H. Structure of the hepatitis C virus IRES bound to the human 80S ribosome: remodeling of the HCV IRES. Structure. 2005 Nov;13(11):1695-706. PMID:16271893 doi:10.1016/j.str.2005.08.008

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2agn"

@@ Line 3: / Line 3: @@
 <SX load='2agn' size='340' side='right' viewer='molstar' caption='[[2agn]], [[Resolution|resolution]] 15.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2agn]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AGN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2agn]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AGN FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2agn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2agn OCA], [https://pdbe.org/2agn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2agn RCSB], [https://www.ebi.ac.uk/pdbsum/2agn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2agn ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 15&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2agn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2agn OCA], [https://pdbe.org/2agn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2agn RCSB], [https://www.ebi.ac.uk/pdbsum/2agn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2agn ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 20: / Line 21: @@
 </SX>
 [[Category: Large Structures]]
-[[Category: Boehringer, D]]
+[[Category: Synthetic construct]]
-[[Category: Lewis, J D]]
+[[Category: Boehringer D]]
-[[Category: Ostareck-Lederer, A]]
+[[Category: Lewis JD]]
-[[Category: Stark, H]]
+[[Category: Ostareck-Lederer A]]
-[[Category: Thermann, R]]
+[[Category: Stark H]]
-[[Category: Hcv]]
+[[Category: Thermann R]]
-[[Category: Ire]]
-[[Category: Rna]]

2agn

From Proteopedia

Revision as of 18:07, 18 October 2023

Fitting of hepatitis C virus internal ribosome entry site domains into the 15 A Cryo-EM map of a HCV IRES-80S ribosome (H. sapiens) complex

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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