2xyy

From Proteopedia

(Difference between revisions)

Revision as of 18:17, 18 October 2023

De Novo model of Bacteriophage P22 procapsid coat protein

2xyy, resolution 3.80Å

Structural highlights

2xyy is a 7 chain structure with sequence from Salmonella virus P22. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.8Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPSD_BPP22 Assembles to form an icosahedral capsid with a T=7 symmetry.

Publication Abstract from PubMed

Formation of many dsDNA viruses begins with the assembly of a procapsid, containing scaffolding proteins and a multisubunit portal but lacking DNA, which matures into an infectious virion. This process, conserved among dsDNA viruses such as herpes viruses and bacteriophages, is key to forming infectious virions. Bacteriophage P22 has served as a model system for this study in the past several decades. However, how capsid assembly is initiated, where and how scaffolding proteins bind to coat proteins in the procapsid, and the conformational changes upon capsid maturation still remain elusive. Here, we report Calpha backbone models for the P22 procapsid and infectious virion derived from electron cryomicroscopy density maps determined at 3.8- and 4.0-A resolution, respectively, and the first procapsid structure at subnanometer resolution without imposing symmetry. The procapsid structures show the scaffolding protein interacting electrostatically with the N terminus (N arm) of the coat protein through its C-terminal helix-loop-helix motif, as well as unexpected interactions between 10 scaffolding proteins and the 12-fold portal located at a unique vertex. These suggest a critical role for the scaffolding proteins both in initiating the capsid assembly at the portal vertex and propagating its growth on a T = 7 icosahedral lattice. Comparison of the procapsid and the virion backbone models reveals coordinated and complex conformational changes. These structural observations allow us to propose a more detailed molecular mechanism for the scaffolding-mediated capsid assembly initiation including portal incorporation, release of scaffolding proteins upon DNA packaging, and maturation into infectious virions.

Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus.,Chen DH, Baker ML, Hryc CF, Dimaio F, Jakana J, Wu W, Dougherty M, Haase-Pettingell C, Schmid MF, Jiang W, Baker D, King JA, Chiu W Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1355-60. Epub 2011 Jan 10. PMID:21220301^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen DH, Baker ML, Hryc CF, Dimaio F, Jakana J, Wu W, Dougherty M, Haase-Pettingell C, Schmid MF, Jiang W, Baker D, King JA, Chiu W. Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1355-60. Epub 2011 Jan 10. PMID:21220301 doi:10.1073/pnas.1015739108

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xyy"

@@ Line 3: / Line 3: @@
 <SX load='2xyy' size='340' side='right' viewer='molstar' caption='[[2xyy]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xyy]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XYY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xyy]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XYY FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xyz|2xyz]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xyy OCA], [https://pdbe.org/2xyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xyy RCSB], [https://www.ebi.ac.uk/pdbsum/2xyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xyy ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CAPSD_BPP22 CAPSD_BPP22]] Assembles to form an icosahedral capsid with a T=7 symmetry.
+[https://www.uniprot.org/uniprot/CAPSD_BPP22 CAPSD_BPP22] Assembles to form an icosahedral capsid with a T=7 symmetry.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </SX>
-[[Category: Bpp22]]
 [[Category: Large Structures]]
-[[Category: Baker, D]]
+[[Category: Salmonella virus P22]]
-[[Category: Baker, M L]]
+[[Category: Baker D]]
-[[Category: Chen, D H]]
+[[Category: Baker ML]]
-[[Category: Chiu, W]]
+[[Category: Chen D-H]]
-[[Category: DiMaio, F]]
+[[Category: Chiu W]]
-[[Category: Dougherty, M]]
+[[Category: DiMaio F]]
-[[Category: Haase-Pettingell, C]]
+[[Category: Dougherty M]]
-[[Category: Hryc, C F]]
+[[Category: Haase-Pettingell C]]
-[[Category: Jakana, J]]
+[[Category: Hryc CF]]
-[[Category: Jiang, W]]
+[[Category: Jakana J]]
-[[Category: King, J A]]
+[[Category: Jiang W]]
-[[Category: Schmid, M F]]
+[[Category: King JA]]
-[[Category: Wu, W]]
+[[Category: Schmid MF]]
-[[Category: Icosahedral reconstruction]]
+[[Category: Wu W]]
-[[Category: Virus]]

2xyy

From Proteopedia

Revision as of 18:17, 18 October 2023

De Novo model of Bacteriophage P22 procapsid coat protein

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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