|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Gp5.7 mutant L42A== | | ==Gp5.7 mutant L42A== |
| - | <StructureSection load='5lgm' size='340' side='right' caption='[[5lgm]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='5lgm' size='340' side='right'caption='[[5lgm]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lgm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lgm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LGM FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">5.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgm OCA], [http://pdbe.org/5lgm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgm RCSB], [http://www.ebi.ac.uk/pdbsum/5lgm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgm ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgm OCA], [https://pdbe.org/5lgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lgm RCSB], [https://www.ebi.ac.uk/pdbsum/5lgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/V5557_BPT7 V5557_BPT7] |
| - | Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic beta and beta' subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the sigma70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.
| + | |
| - | | + | |
| - | A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement.,Liu B, Shadrin A, Sheppard C, Mekler V, Xu Y, Severinov K, Matthews S, Wigneshweraraj S Nucleic Acids Res. 2014 Jan 30. PMID:24482445<ref>PMID:24482445</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5lgm" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpt7]] | + | [[Category: Escherichia phage T7]] |
| - | [[Category: Liu, B]] | + | [[Category: Large Structures]] |
| - | [[Category: Matthews, S]] | + | [[Category: Liu B]] |
| - | [[Category: Anti-sigma effector]] | + | [[Category: Matthews S]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription effector]]
| + | |
