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5lon

From Proteopedia

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Structure of /K. lactis/ Dcp1-Dcp2 decapping complex.

Structural highlights

5lon is a 2 chain structure with sequence from Kluyveromyces lactis NRRL Y-1140. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.5Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q6CIU1_KLULA

Publication Abstract from PubMed

Elimination of the 5' cap of eukaryotic mRNAs, known as decapping, is considered to be a crucial, irreversible and highly regulated step required for the rapid degradation of mRNA by Xrn1, the major cytoplasmic 5'-3' exonuclease. Decapping is accomplished by the recruitment of a protein complex formed by the Dcp2 catalytic subunit and its Dcp1 cofactor. However, this complex has a low intrinsic enzymatic activity and requires several accessory proteins such as the Lsm1-7 complex, Pat1, Edc1-Edc2 and/or Edc3 to be fully active. Here we present the crystal structure of the active form of the yeast Kluyveromyces lactis Dcp1-Dcp2 enzyme bound to its product (m7GDP) and its potent activator Edc3. This structure of the Dcp1-Dcp2 complex bound to a cap analog further explains previously published data on substrate binding and provides hints as to the mechanism of Edc3-mediated Dcp2 activation.

Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator.,Charenton C, Taverniti V, Gaudon-Plesse C, Back R, Seraphin B, Graille M Nat Struct Mol Biol. 2016 Oct 3. doi: 10.1038/nsmb.3300. PMID:27694841^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Charenton C, Taverniti V, Gaudon-Plesse C, Back R, Seraphin B, Graille M. Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator. Nat Struct Mol Biol. 2016 Oct 3. doi: 10.1038/nsmb.3300. PMID:27694841 doi:http://dx.doi.org/10.1038/nsmb.3300

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lon"

@@ Line 3: / Line 3: @@
 <StructureSection load='5lon' size='340' side='right'caption='[[5lon]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lon]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LON OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LON FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lon]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LON FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0_F23980g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_E01827g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lon OCA], [http://pdbe.org/5lon PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lon RCSB], [http://www.ebi.ac.uk/pdbsum/5lon PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lon ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lon OCA], [https://pdbe.org/5lon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lon RCSB], [https://www.ebi.ac.uk/pdbsum/5lon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lon ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q6CIU1_KLULA Q6CIU1_KLULA]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Candida sphaerica]]
+[[Category: Kluyveromyces lactis NRRL Y-1140]]
 [[Category: Large Structures]]
-[[Category: Back, R]]
+[[Category: Back R]]
-[[Category: Charenton, C]]
+[[Category: Charenton C]]
-[[Category: Gaudon-Plesse, C]]
+[[Category: Gaudon-Plesse C]]
-[[Category: Graille, M]]
+[[Category: Graille M]]
-[[Category: Seraphin, B]]
+[[Category: Seraphin B]]
-[[Category: Taverniti, V]]
+[[Category: Taverniti V]]
-[[Category: Multiprotein complex]]
-[[Category: Rna binding protein]]
-[[Category: Rna decay]]

5lon

From Proteopedia

Current revision

Structure of /K. lactis/ Dcp1-Dcp2 decapping complex.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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