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| | <StructureSection load='5lop' size='340' side='right'caption='[[5lop]], [[Resolution|resolution]] 3.50Å' scene=''> | | <StructureSection load='5lop' size='340' side='right'caption='[[5lop]], [[Resolution|resolution]] 3.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lop]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LOP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LOP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lop]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LOP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5-DIPHOSPHATE'>M7G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0_F23980g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_E01827g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_A11308g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5-DIPHOSPHATE'>M7G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lop OCA], [http://pdbe.org/5lop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lop RCSB], [http://www.ebi.ac.uk/pdbsum/5lop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lop ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lop OCA], [https://pdbe.org/5lop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lop RCSB], [https://www.ebi.ac.uk/pdbsum/5lop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lop ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q6CPV9_KLULA Q6CPV9_KLULA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Candida sphaerica]] | + | [[Category: Kluyveromyces lactis NRRL Y-1140]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Back, R]] | + | [[Category: Back R]] |
| - | [[Category: Charenton, C]] | + | [[Category: Charenton C]] |
| - | [[Category: Gaudon-Plesse, C]] | + | [[Category: Gaudon-Plesse C]] |
| - | [[Category: Graille, M]] | + | [[Category: Graille M]] |
| - | [[Category: Seraphin, B]] | + | [[Category: Seraphin B]] |
| - | [[Category: Taverniti, V]] | + | [[Category: Taverniti V]] |
| - | [[Category: Multiprotein complex]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna decay]]
| + | |
| Structural highlights
Function
Q6CPV9_KLULA
Publication Abstract from PubMed
Elimination of the 5' cap of eukaryotic mRNAs, known as decapping, is considered to be a crucial, irreversible and highly regulated step required for the rapid degradation of mRNA by Xrn1, the major cytoplasmic 5'-3' exonuclease. Decapping is accomplished by the recruitment of a protein complex formed by the Dcp2 catalytic subunit and its Dcp1 cofactor. However, this complex has a low intrinsic enzymatic activity and requires several accessory proteins such as the Lsm1-7 complex, Pat1, Edc1-Edc2 and/or Edc3 to be fully active. Here we present the crystal structure of the active form of the yeast Kluyveromyces lactis Dcp1-Dcp2 enzyme bound to its product (m7GDP) and its potent activator Edc3. This structure of the Dcp1-Dcp2 complex bound to a cap analog further explains previously published data on substrate binding and provides hints as to the mechanism of Edc3-mediated Dcp2 activation.
Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator.,Charenton C, Taverniti V, Gaudon-Plesse C, Back R, Seraphin B, Graille M Nat Struct Mol Biol. 2016 Oct 3. doi: 10.1038/nsmb.3300. PMID:27694841[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Charenton C, Taverniti V, Gaudon-Plesse C, Back R, Seraphin B, Graille M. Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator. Nat Struct Mol Biol. 2016 Oct 3. doi: 10.1038/nsmb.3300. PMID:27694841 doi:http://dx.doi.org/10.1038/nsmb.3300
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