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| | <StructureSection load='5lsb' size='340' side='right'caption='[[5lsb]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5lsb' size='340' side='right'caption='[[5lsb]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lsb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LSB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lsb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LSB FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HSH49, YOR319W, O6142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), CUS1, YMR240C, YM9408.02C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsb OCA], [http://pdbe.org/5lsb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsb RCSB], [http://www.ebi.ac.uk/pdbsum/5lsb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsb OCA], [https://pdbe.org/5lsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lsb RCSB], [https://www.ebi.ac.uk/pdbsum/5lsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HSH49_YEAST HSH49_YEAST]] Possible SF3b-like factor. [[http://www.uniprot.org/uniprot/CUS1_YEAST CUS1_YEAST]] Essential splicing protein required for U2 snRNP binding to pre-mRNA during spliceosome assembly. | + | [https://www.uniprot.org/uniprot/HSH49_YEAST HSH49_YEAST] Possible SF3b-like factor. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Nagai, K]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Obayashi, E]] | + | [[Category: Nagai K]] |
| - | [[Category: Oubridge, C]] | + | [[Category: Obayashi E]] |
| - | [[Category: Roon, A M.van]] | + | [[Category: Oubridge C]] |
| - | [[Category: Sposito, B]] | + | [[Category: Sposito B]] |
| - | [[Category: Rna binding domain]] | + | [[Category: Van Roon AM]] |
| - | [[Category: Sf3b complex]]
| + | |
| - | [[Category: Splicing]]
| + | |
| - | [[Category: U2 snrnp]]
| + | |
| Structural highlights
Function
HSH49_YEAST Possible SF3b-like factor.
Publication Abstract from PubMed
Spliceosomal proteins Hsh49p and Cus1p are components of SF3b, which together with SF3a, Msl1p/Lea1p, Sm proteins and U2 snRNA, form U2 snRNP, which plays a crucial role in pre-mRNA splicing. Hsh49p, comprising two RRMs, forms a heterodimer with Cus1p. We determined the crystal structures of Saccharomyces cerevisiae full-length Hsh49p as well as its RRM1 in complex with a minimal binding region of Cus1p (residues 290-368). The structures show that the Cus1 fragment binds to the alpha-helical surface of Hsh49p RRM1, opposite the four-stranded beta-sheet, leaving the canonical RNA binding surface available to bind RNA. Hsh49p binds the 5' end region of U2 snRNA via RRM1. Its affinity is increased in complex with Cus1(290-368)p, partly because an extended RNA-binding surface forms across the protein-protein interface. The Hsh49p RRM1-Cus1(290-368)p structure fits well into cryo-EM density of the Bact spliceosome, corroborating the biological relevance of our crystal structure.
Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p binding domain.,van Roon AA, Oubridge C, Obayashi E, Sposito B, Newman AJ, Seraphin B, Nagai K RNA. 2017 Mar 27. pii: rna.059378.116. doi: 10.1261/rna.059378.116. PMID:28348170[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ van Roon AA, Oubridge C, Obayashi E, Sposito B, Newman AJ, Seraphin B, Nagai K. Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p binding domain. RNA. 2017 Mar 27. pii: rna.059378.116. doi: 10.1261/rna.059378.116. PMID:28348170 doi:http://dx.doi.org/10.1261/rna.059378.116
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