1not
From Proteopedia
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'''THE 1.2 ANGSTROM STRUCTURE OF G1 ALPHA CONOTOXIN''' | '''THE 1.2 ANGSTROM STRUCTURE OF G1 ALPHA CONOTOXIN''' | ||
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[[Category: Martin, J L.]] | [[Category: Martin, J L.]] | ||
[[Category: Shan, L.]] | [[Category: Shan, L.]] | ||
| - | [[Category: | + | [[Category: Acetylcholine receptor]] |
| - | [[Category: | + | [[Category: Conotoxin]] |
| - | [[Category: | + | [[Category: Disulphide loop]] |
| - | [[Category: | + | [[Category: Venom]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:47:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:47, 2 May 2008
THE 1.2 ANGSTROM STRUCTURE OF G1 ALPHA CONOTOXIN
Overview
Predatory marine snails of the genus Conus paralyze their fish prey by injecting a potent toxin. The alpha-conotoxin GI is a 13-residue peptide isolated from venom of Conus geographus. It functions by blocking the postsynaptic nicotinic acetylcholine receptor. After crystallization in deionized water, the three-dimensional structure of the GI neurotoxin was determined to 1.2 A resolution by X-ray crystallography. This structure, which can be described as a triangular slab, shows overall similarities to those derived by NMR, CD, and predictive methods. The principal framework of the molecule is provided by two disulfide bonds, one linking Cys 2 and Cys 7 and the other Cys 3 and Cys 13. Opposite ends of the sequence are drawn together even further by hydrogen bonds between Glu 1 and Cys 13 and between Cys 2 and Ser 12. Since the C-terminus is amidated, only one negative charge is present (carboxylate of Glu 1), and this is not implicated in receptor binding. Two positively charged regions (the alpha-amino group of Glu 1 and the guanido group of Arg 9) are situated 15 A apart at the corners of the triangular face of the molecule. phi, psi angles characteristic of a 3(10) helix were observed for residues 5-7. For residues 8-11, these angles were consistent with either a type I beta-turn or a distorted 3(10) helix.
About this Structure
1NOT is a Single protein structure of sequence from Conus geographus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the alpha-conotoxin GI at 1.2 A resolution., Guddat LW, Martin JA, Shan L, Edmundson AB, Gray WR, Biochemistry. 1996 Sep 3;35(35):11329-35. PMID:8784187 Page seeded by OCA on Sat May 3 02:47:41 2008
