1np3
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1np3.jpg|left|200px]] | [[Image:1np3.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1np3", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1np3| PDB=1np3 | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa''' | '''Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa''' | ||
| Line 32: | Line 29: | ||
[[Category: Suh, S W.]] | [[Category: Suh, S W.]] | ||
[[Category: Yoon, H J.]] | [[Category: Yoon, H J.]] | ||
| - | [[Category: | + | [[Category: A deep figure-of-eight knot]] |
| - | [[Category: | + | [[Category: C-terminal alpha-helical domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:48:13 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:48, 2 May 2008
Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa
Overview
Acetohydroxy acid isomeroreductase (AHIR) is a key enzyme in the biosynthesis of branched-chain amino acids. We have determined the first crystal structure of a class I AHIR from Pseudomonas aeruginosa at 2.0 A resolution. Its dodecameric architecture of 23 point group symmetry is assembled of six dimeric units and dimerization is essential for the formation of the active site. The dimeric unit of P.aeruginosa AHIR partially superimposes with a three-domain monomer of spinach AHIR, a class II enzyme. This demonstrates that the so-called plant-specific insert in the middle of spinach AHIR is structurally and functionally equivalent to the C-terminal alpha-helical domain of P.aeruginosa AHIR, and the C-terminal alpha-helical domain was duplicated during evolution from the shorter, class I AHIRs to the longer, class II AHIRs. The dimeric unit of P.aeruginosa AHIR possesses a deep figure-of-eight knot, essentially identical with that in the spinach AHIR monomer. Thus, our work lowers the likelihood of the previous proposal that "domain duplication followed by exchange of a secondary structure element can be a source of such a knot in the protein structure" being correct.
About this Structure
1NP3 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Crystal structure of class I acetohydroxy acid isomeroreductase from Pseudomonas aeruginosa., Ahn HJ, Eom SJ, Yoon HJ, Lee BI, Cho H, Suh SW, J Mol Biol. 2003 Apr 25;328(2):505-15. PMID:12691757 Page seeded by OCA on Sat May 3 02:48:13 2008
