1np9
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1np9.gif|left|200px]] | [[Image:1np9.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1np9", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1np9| PDB=1np9 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of the parallel-stranded DNA quadruplex d(TTAGGGA)4 containing the human telomeric repeat''' | '''Structure of the parallel-stranded DNA quadruplex d(TTAGGGA)4 containing the human telomeric repeat''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NP9 OCA]. | |
==Reference== | ==Reference== | ||
Structure of the parallel-stranded DNA quadruplex d(TTAGGGT)4 containing the human telomeric repeat: evidence for A-tetrad formation from NMR and molecular dynamics simulations., Gavathiotis E, Searle MS, Org Biomol Chem. 2003 May 21;1(10):1650-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12926351 12926351] | Structure of the parallel-stranded DNA quadruplex d(TTAGGGT)4 containing the human telomeric repeat: evidence for A-tetrad formation from NMR and molecular dynamics simulations., Gavathiotis E, Searle MS, Org Biomol Chem. 2003 May 21;1(10):1650-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12926351 12926351] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Gavathiotis, E.]] | [[Category: Gavathiotis, E.]] | ||
[[Category: Searle, M S.]] | [[Category: Searle, M S.]] | ||
| - | [[Category: | + | [[Category: A-tetrad]] |
| - | [[Category: | + | [[Category: Parallel-stranded quadruplex dna]] |
| - | [[Category: | + | [[Category: Ttagggt repeat]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:48:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:48, 2 May 2008
Structure of the parallel-stranded DNA quadruplex d(TTAGGGA)4 containing the human telomeric repeat
Overview
The structure of the intermolecular DNA quadruplex d(TTAGGGT)4, based on the human telomeric DNA sequence d(TTAGGG), has been determined in solution by NMR and restrained molecular dynamics simultations. The core GGG region forms a highly stable quadruplex with G-tetrads likely stabilised by K+ ions bound between tetrad plains. However, we have focused on the conformation of the adenines which differ considerably in base alignment, stability and dynamics from those in previously reported structures of d(AGGGT)4 and d(TAGGGT)4. We show unambiguously that the adenines of d(TTAGGGT)4 are involved in the formation of a relatively stable A-tetrad with well-defined glycosidic torsion angles (anti), hydrogen bonding network (adenine 6-NH2-adenine N1) defined by interbase NOEs, and base stacking interactions with the neighbouring G-tetrad. All of these structural features are apparent from NOE data involving both exchangeable and non-exchangeable protons. Thus, context-dependent effects appear to play some role in dictating preferred conformation, stability and dynamics. The structure of d(TTAGGGT)4 provides us with a model system for exploiting in the design of novel telomerase inhibitors that bind to and stabilise G-quadruplex structures.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of the parallel-stranded DNA quadruplex d(TTAGGGT)4 containing the human telomeric repeat: evidence for A-tetrad formation from NMR and molecular dynamics simulations., Gavathiotis E, Searle MS, Org Biomol Chem. 2003 May 21;1(10):1650-6. PMID:12926351 Page seeded by OCA on Sat May 3 02:48:36 2008
