1npb
From Proteopedia
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'''Crystal structure of the fosfomycin resistance protein from transposon Tn2921''' | '''Crystal structure of the fosfomycin resistance protein from transposon Tn2921''' | ||
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[[Category: Pakhomova, S.]] | [[Category: Pakhomova, S.]] | ||
[[Category: Rife, C L.]] | [[Category: Rife, C L.]] | ||
| - | [[Category: | + | [[Category: Manganese binding]] |
| - | [[Category: | + | [[Category: Potassium binding loop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:48:48 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:48, 2 May 2008
Crystal structure of the fosfomycin resistance protein from transposon Tn2921
Overview
The crystal structure of fosfomycin resistance protein FosA from transposon Tn2921 has been established at a resolution of 2.5 A. The protein crystallized without bound Mn(II) and K+, ions crucial for efficient catalysis, providing a structure of the apo enzyme. The protein maintains the three-dimensional domain-swapped arrangement of the paired betaalphabetabetabeta-motifs observed in the genomically encoded homologous enzyme from Pseudomonas aeruginosa (PA1129). The basic architecture of the active site is also maintained, despite the absence of the catalytically essential Mn(II). However, the absence of K+, which has been shown to enhance enzymatic activity, appears to contribute to conformational heterogeneity in the K(+)-binding loops.
About this Structure
1NPB is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.
Reference
Structure of fosfomycin resistance protein FosA from transposon Tn2921., Pakhomova S, Rife CL, Armstrong RN, Newcomer ME, Protein Sci. 2004 May;13(5):1260-5. Epub 2004 Apr 9. PMID:15075406 Page seeded by OCA on Sat May 3 02:48:48 2008
