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| | <StructureSection load='1rj1' size='340' side='right'caption='[[1rj1]], [[Resolution|resolution]] 1.87Å' scene=''> | | <StructureSection load='1rj1' size='340' side='right'caption='[[1rj1]], [[Resolution|resolution]] 1.87Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1rj1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RJ1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1rj1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RJ1 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rj4|1rj4]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NTINH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rj1 OCA], [https://pdbe.org/1rj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rj1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rj1 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rj1 OCA], [http://pdbe.org/1rj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rj1 RCSB], [http://www.ebi.ac.uk/pdbsum/1rj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rj1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O49908_TOBAC O49908_TOBAC] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: American tobacco]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Angelo, I D]] | + | [[Category: Nicotiana tabacum]] |
| - | [[Category: Greiner, S]] | + | [[Category: D'Angelo I]] |
| - | [[Category: Hothorn, M]] | + | [[Category: Greiner S]] |
| - | [[Category: Marquez, J A]] | + | [[Category: Hothorn M]] |
| - | [[Category: Scheffzek, K]] | + | [[Category: Marquez JA]] |
| - | [[Category: Four-helix bundle]]
| + | [[Category: Scheffzek K]] |
| - | [[Category: Helical hairpin]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Function
O49908_TOBAC
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Plant invertases are sucrolytic enzymes essential for plant metabolism and development. Enzyme activity is regulated on a posttranslational level via inhibitory proteins, referred to as invertase inhibitors. Ectopic expression of invertase inhibitors in crop plants has high biotechnological potential. However, little biochemical and up to now no detailed structural information is available about this class of plant regulatory proteins. Here, we present the crystal structure of the cell wall-associated invertase inhibitor Nt-CIF from tobacco at a resolution of 1.87A. The structural model reveals an asymmetric four-helix bundle with an uncommon N-terminal extension that appears to be critical for the structural integrity of the protein. Structure analysis of a second crystal form grown in the presence of CdCl(2) reveals two metal binding sites. Nt-CIF is highly thermostable and retains full inhibitory activity after cooling to ambient temperatures. The structure of Nt-CIF provides the first three-dimensional information source for the posttranslational regulation of plant invertases. Based on the recently discovered sequence homology between inhibitors of invertases and pectin methylesterases, our structural model is likely to represent a scaffold also used for the regulation of the latter enzymes, which do not share sequence similarity with invertases. Thus, our structural model sets the 3D-stage for the investigation of posttranslational regulation of invertases as well as pectin methylesterases.
The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension.,Hothorn M, D'Angelo I, Marquez JA, Greiner S, Scheffzek K J Mol Biol. 2004 Jan 23;335(4):987-95. PMID:14698293[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hothorn M, D'Angelo I, Marquez JA, Greiner S, Scheffzek K. The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension. J Mol Biol. 2004 Jan 23;335(4):987-95. PMID:14698293
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