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| | <StructureSection load='1y1u' size='340' side='right'caption='[[1y1u]], [[Resolution|resolution]] 3.21Å' scene=''> | | <StructureSection load='1y1u' size='340' side='right'caption='[[1y1u]], [[Resolution|resolution]] 3.21Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1y1u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y1U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1y1u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y1U FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y1u OCA], [https://pdbe.org/1y1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y1u RCSB], [https://www.ebi.ac.uk/pdbsum/1y1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y1u ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.21Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y1u OCA], [https://pdbe.org/1y1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y1u RCSB], [https://www.ebi.ac.uk/pdbsum/1y1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y1u ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/STA5A_MOUSE STA5A_MOUSE]] Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.<ref>PMID:10508857</ref> <ref>PMID:16837552</ref>
| + | [https://www.uniprot.org/uniprot/STA5A_MOUSE STA5A_MOUSE] Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.<ref>PMID:10508857</ref> <ref>PMID:16837552</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Becker, S]] | + | [[Category: Becker S]] |
| - | [[Category: Klumpp, K]] | + | [[Category: Klumpp K]] |
| - | [[Category: Neculai, A M]] | + | [[Category: Neculai AM]] |
| - | [[Category: Neculai, D]] | + | [[Category: Neculai D]] |
| - | [[Category: Pfitzner, E]] | + | [[Category: Pfitzner E]] |
| - | [[Category: Straub, K]] | + | [[Category: Straub K]] |
| - | [[Category: Verrier, S]] | + | [[Category: Verrier S]] |
| - | [[Category: Activator]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Sh2 domain]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Stat]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| Structural highlights
Function
STA5A_MOUSE Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
STAT proteins have the function of signaling from the cell membrane into the nucleus, where they regulate gene transcription. Latent mammalian STAT proteins can form dimers in the cytoplasm even before receptor-mediated activation by specific tyrosine phosphorylation. Here we describe the 3.21-A crystal structure of an unphosphorylated STAT5a homodimer lacking the N-terminal domain as well as the C-terminal transactivation domain. The overall structure of this fragment is very similar to phosphorylated STATs. However, important differences exist in the dimerization mode. Although the interface between phosphorylated STATs is mediated by their Src-homology 2 domains, the unphosphorylated STAT5a fragment dimerizes in a completely different manner via interactions between their beta-barrel and four-helix bundle domains. The STAT4 N-terminal domain dimer can be docked onto this STAT5a core fragment dimer based on shape and charge complementarities. The separation of the dimeric arrangement, taking place upon activation and nuclear translocation of STAT5a, is demonstrated by fluorescence resonance energy transfer experiments in living cells.
Structure of the unphosphorylated STAT5a dimer.,Neculai D, Neculai AM, Verrier S, Straub K, Klumpp K, Pfitzner E, Becker S J Biol Chem. 2005 Dec 9;280(49):40782-7. Epub 2005 Sep 28. PMID:16192273[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jones FE, Welte T, Fu XY, Stern DF. ErbB4 signaling in the mammary gland is required for lobuloalveolar development and Stat5 activation during lactation. J Cell Biol. 1999 Oct 4;147(1):77-88. PMID:10508857
- ↑ Muraoka-Cook RS, Sandahl M, Husted C, Hunter D, Miraglia L, Feng SM, Elenius K, Earp HS 3rd. The intracellular domain of ErbB4 induces differentiation of mammary epithelial cells. Mol Biol Cell. 2006 Sep;17(9):4118-29. Epub 2006 Jul 12. PMID:16837552 doi:10.1091/mbc.E06-02-0101
- ↑ Neculai D, Neculai AM, Verrier S, Straub K, Klumpp K, Pfitzner E, Becker S. Structure of the unphosphorylated STAT5a dimer. J Biol Chem. 2005 Dec 9;280(49):40782-7. Epub 2005 Sep 28. PMID:16192273 doi:10.1074/jbc.M507682200
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